Identification of lncRNA DLEU2 as a potential diagnostic biomarker and anti-inflammatory target for ulcerative colitis

Lin, Qiuling1; Zhang, Dingguo2; Zhang, Jian3; Luo, Weixiang4; Xu, Zhenglei2; Yao, Jun2; Wang, Lisheng2

2022-09-14

10.3389/fphar.2022.991448

FRONTIERS IN PHARMACOLOGY   影响因子和分区

1663-9812

The incidence of ulcerative colitis (UC) in China has significantly increased over the past 10 years. Here we aim to explore potential diagnostic biomarkers and anti-inflammatory targets associated with UC. Patients with UC were enrolled in this study. The expression of lncRNAs and mRNAs in the nidus of the gut mucosa and adjacent normal mucosa samples was evaluated by RNA sequencing. The role of DLEU2 in inflammation and NF-kappa B signaling pathway was examined by RT-qPCR, Western blotting, and ELISA with human macrophage-like cells derived from THP-1. 564 lncRNAs and 859 mRNAs are significantly altered in the nidus of the gut mucosa of UC patients. Among the differentially expressed lncRNAs, DLEU2 changes the most. The expression of DLEU2 is negatively associated with inflammatory factors such as TNF-alpha, IL-1 alpha, IL-1 beta, IL-6, and NLRP3. Mechanistically, DLEU2 exerts anti-inflammatory activity by inhibiting the NF-kappa B signaling pathway. In conclusion, the lncRNA DLEU2 in the intestinal mucosa is dysregulated upon gut inflammation and may act as a diagnostic biomarker and a therapeutic target for UC.

ulcerative colitis DLEU2 inflammatory factors NF-kappa B lncRNA

SCI

英语

第一 ; 通讯

[JCYJ20210324113803011] ; [2018A030313900]

Pharmacology & Pharmacy

Pharmacology & Pharmacy

WOS:000861767600001

FRONTIERS MEDIA SA

Web of Science

1.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Gen Practic, Shenzhen, Guangdong, Peoples R China
2.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Gastroenter, Shenzhen, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Plast Surg, Guangzhou, Guangdong, Peoples R China
4.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Nursing, Shenzhen, Guangdong, Peoples R China

Lin, Qiuling,Zhang, Dingguo,Zhang, Jian,et al. Identification of lncRNA DLEU2 as a potential diagnostic biomarker and anti-inflammatory target for ulcerative colitis[J]. FRONTIERS IN PHARMACOLOGY,2022,13.

Lin, Qiuling.,Zhang, Dingguo.,Zhang, Jian.,Luo, Weixiang.,Xu, Zhenglei.,...&Wang, Lisheng.(2022).Identification of lncRNA DLEU2 as a potential diagnostic biomarker and anti-inflammatory target for ulcerative colitis.FRONTIERS IN PHARMACOLOGY,13.

Lin, Qiuling,et al."Identification of lncRNA DLEU2 as a potential diagnostic biomarker and anti-inflammatory target for ulcerative colitis".FRONTIERS IN PHARMACOLOGY 13(2022).