南方科技大学知识苑(SUSTech KC): 3D printing of reduced glutathione grafted gelatine methacrylate hydrogel scaffold promotes diabetic bone regeneration by activating PI3K/Akt signaling pathway

题名	3D printing of reduced glutathione grafted gelatine methacrylate hydrogel scaffold promotes diabetic bone regeneration by activating PI3K/Akt signaling pathway
作者	Wang，Lulu1 ; Shen，Mingkui1 ; Hou，Qiaodan1 ; Wu，Zimei1 ; Xu，Jing 2 ; Wang，Lin1,2
通讯作者	Wang，Lin
发表日期	2022-12-01
DOI	10.1016/j.ijbiomac.2022.09.236
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 影响因子和分区
ISSN	0141-8130
EISSN	1879-0003
卷号	222页码:1175-1191
摘要	Diabetic individuals are frequently associated with increased fracture risk and poor bone healing capacity, and the treatment of diabetic bone defects remains a great challenge in orthopedics. In this study, an antioxidant hydrogel was developed using reduced glutathione grafted gelatine methacrylate (GelMA-g-GSH), followed by 3D printing to form a tissue engineering scaffold, which possessed appropriate mechanical property and good biocompatibility. In vitro studies displayed that benefitting from the sustained delivery of reduced glutathione, GelMA-g-GSH scaffold enabled to suppress the overproduction of reactive oxygen species (ROS) and reduce the oxidative stress of cells. Osteogenic experiments showed that GelMA-g-GSH scaffold exhibited excellent osteogenesis performance, with the elevated expression levels of osteogenesis-related genes and proteins. Further, RNA-sequencing revealed that activation of PI3K/Akt signaling pathway of MC3T3-E1 seeded on GelMA-g-GSH scaffold may be the underlying mechanism in promoting osteogenesis. In vivo, diabetic mice calvarial defects experiment demonstrated enhanced bone regeneration after the implantation of GelMA-g-GSH scaffold, as shown by micro-CT and histological analysis. In summary, 3D-printed GelMA-g-GSH scaffold can not only scavenge ROS, but also promote proliferation and differentiation of osteoblasts by activating PI3K/Akt signaling pathway, thereby accelerating bone repair under diabetes.
关键词	3D printing Antioxidation Diabetic bone regeneration GelMA-g-GSH RNA sequencing analysis
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Basic and Applied Basic Research Foundation of Guangdong Province[2022A1515012373];National Natural Science Foundation of China[81972045];
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000867226000002
出版者	ELSEVIER
ESI学科分类	BIOLOGY & BIOCHEMISTRY
Scopus记录号	2-s2.0-85139355609
来源库	Scopus
引用统计	被引频次[WOS]：24
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/406160
专题	南方科技大学医学院南方科技大学医院
作者单位	1.School of Medicine,Southern University of Science and Technology,Shenzhen,No. 1088 Xueyuan Avenue, Guangdong Province,518055,China 2.Southern University of Science and Technology Hospital,Shenzhen,6019 Liuxian Avenue,518055,China
第一作者单位	南方科技大学医学院
通讯作者单位	南方科技大学医学院; 南方科技大学医院
第一作者的第一单位	南方科技大学医学院
推荐引用方式 GB/T 7714	Wang，Lulu,Shen，Mingkui,Hou，Qiaodan,et al. 3D printing of reduced glutathione grafted gelatine methacrylate hydrogel scaffold promotes diabetic bone regeneration by activating PI3K/Akt signaling pathway[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,222:1175-1191.
APA	Wang，Lulu,Shen，Mingkui,Hou，Qiaodan,Wu，Zimei,Xu，Jing,&Wang，Lin.(2022).3D printing of reduced glutathione grafted gelatine methacrylate hydrogel scaffold promotes diabetic bone regeneration by activating PI3K/Akt signaling pathway.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,222,1175-1191.
MLA	Wang，Lulu,et al."3D printing of reduced glutathione grafted gelatine methacrylate hydrogel scaffold promotes diabetic bone regeneration by activating PI3K/Akt signaling pathway".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 222(2022):1175-1191.