Cefazolin/BMP-2-Loaded Mesoporous Silica Nanoparticles for the Repair of Open Fractures with Bone Defects

Shen，Mingkui; Wang，Lulu; Feng，Li; Xu，Chuangye; Gao，Yi; Li，Sijing; Wu，Yulan; Pei，Guoxian

2022

10.1155/2022/8385456

Oxidative Medicine and Cellular Longevity   影响因子和分区

1942-0900

1942-0994

The study aimed to explore the feasibility of a nanodrug delivery system to treat open fractures with bone defects. We developed a cefazolin (Cef)/bone morphogenetic protein 2 (BMP-2)@mesoporous silica nanoparticle (MSN) delivery system; meanwhile, Cef/MBP-2@ poly(lactic-co-glycolic acid) (PLGA) was also developed as control. For the purpose of determining the osteogenic and anti-inflammatory actions of the nanodelivery system, we cultured bone marrow mesenchymal stem cells (BMSCs) and constructed a bone defect mouse model to evaluate its clinical efficacy. After physicochemical property testing, we determined that MSN had good stability and did not easily accumulate or precipitate and it could effectively prolong the Cef's half-life by nearly eight times. In BMSCs, we found that compared with the PLGA delivery system, MSNs better penetrated into the bone tissue, thus effectively increasing BMSCs' proliferation and migration ability to facilitate bone defect repair. Furthermore, the MSN delivery system could improve BMSCs' mineralization indexes (alkaline phosphatase [ALP], osteocalcin [OCN], and collagen I [Col I]) to effectively improve its osteogenic ability. Moreover, the MSN delivery system could inhibit inflammation in bone defect mice, which was mainly reflected in its ability to reduce the release of IL-1ß and IL-4 and increase IL-10 levels; it could also effectively reduce apoptosis of CD4+ and CD8+ T cells, thus improving their immune function. Furthermore, the percentage of new bones, bone mineral density, trabecular volume, and trabecular numbers in the fracture region were improved in mice treated with MSN, which allowed better repair of bone defects. Hence, Cef/BMP-2@MSN may be feasible for open fractures with bone defects.

SCI

英语

第一 ; 通讯

[Y01416214]

Cell Biology

Cell Biology

WOS:000892079100002

HINDAWI LTD

2-s2.0-85139140761

Scopus

School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Shen，Mingkui,Wang，Lulu,Feng，Li,et al. Cefazolin/BMP-2-Loaded Mesoporous Silica Nanoparticles for the Repair of Open Fractures with Bone Defects[J]. Oxidative Medicine and Cellular Longevity,2022,2022.

Shen，Mingkui.,Wang，Lulu.,Feng，Li.,Xu，Chuangye.,Gao，Yi.,...&Pei，Guoxian.(2022).Cefazolin/BMP-2-Loaded Mesoporous Silica Nanoparticles for the Repair of Open Fractures with Bone Defects.Oxidative Medicine and Cellular Longevity,2022.

Shen，Mingkui,et al."Cefazolin/BMP-2-Loaded Mesoporous Silica Nanoparticles for the Repair of Open Fractures with Bone Defects".Oxidative Medicine and Cellular Longevity 2022(2022).