南方科技大学知识苑(SUSTech KC): Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages

题名	Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages
作者	Xiao, Guohui 1; Zhang, Su 1; Zhang, Like 2; Liu, Shuyan 1; Li, Guobao 1; Ou, Min 1; Zeng, Xuan 1; Wang, Zhaoqin 1; Zhang, Guoliang 1,2; Lu, Shuihua 1
通讯作者	Wang, Zhaoqin; Zhang, Guoliang; Lu, Shuihua
发表日期	2022-10-17
DOI	10.1186/s12866-022-02659-y
发表期刊	BMC MICROBIOLOGY 影响因子和分区
ISSN	1471-2180
卷号	22 期号:1
摘要	Background Tuberculosis (TB) caused by Mycobacterium tuberculosis (M. tb) remains a global health issue. The characterized virulent M. tb H37Rv, avirulent M. tb H37Ra and BCG strains are widely used as reference strains to investigate the mechanism of TB pathogenicity. Here, we attempted to determine metabolomic signatures associated with the Mycobacterial virulence in human macrophages through comparison of metabolite profile in THP-1-derived macrophages following exposure to the M. tb H37Rv, M. tb H37Ra and BCG strains. Results Our findings revealed remarkably changed metabolites in infected macrophages compared to uninfected macrophages. H37Rv infection specifically induced 247 differentially changed metabolites compared to H37Ra or BCG infection. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed H37Rv specifically induces tryptophan metabolism. Moreover, quantitative PCR (qPCR) results showed that indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase 2 (TDO2) which converts the tryptophan to a series of biologically second metabolites were up-regulated in H37Rv-infected macrophages compared to H37Ra- or BCG-infected macrophages, confirming the result of enhanced tryptophan metabolism induced by H37Rv infection. These findings indicated that targeting tryptophan (Trp) metabolism may be a potential therapeutic strategy for pulmonary TB. Conclusions We identified a number of differentially changed metabolites that specifically induced in H37Rv infected macrophages. These signatures may be associated with the Mycobacterial virulence in human macrophages. The present findings provide a better understanding of the host response associated with the virulence of the Mtb strain.
关键词	mycobacterium tuberculosis Metabolome THP-1 Tryptophan metabolism Virulence
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Key Research and Development Plan["2021YFA1300902","2020YFA0907201"] ; National Natural Science Foundation of China[82170009] ; Guangdong Scientific and Technological Foundation["2019B1515120041","2020B1111170014","2020A1515010977"] ; Shenzhen Scientific and Technological Foundation[KCXFZ202002011007083]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000869251300001
出版者	BMC
ESI学科分类	MICROBIOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/406504
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Guangdong Prov Clin Res Ctr TB, Natl Clin Res Ctr Infect Dis, Shenzhen 518112, Peoples R China 2.Guangdong Med Univ, Sch Basic Med Sci, Dongguan, Peoples R China
第一作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Xiao, Guohui,Zhang, Su,Zhang, Like,et al. Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages[J]. BMC MICROBIOLOGY,2022,22(1).
APA	Xiao, Guohui.,Zhang, Su.,Zhang, Like.,Liu, Shuyan.,Li, Guobao.,...&Lu, Shuihua.(2022).Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages.BMC MICROBIOLOGY,22(1).
MLA	Xiao, Guohui,et al."Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages".BMC MICROBIOLOGY 22.1(2022).