南方科技大学知识苑(SUSTech KC): Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer

题名	Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer
作者	Wang, Daxuan 1; Feng, Ziyang 2; Lin, Changjian 1; Xie, Tianjian 3; Li, Wujin 4; Wu, Famiao 3; Chen, Zhengwei 1; Wang, Yan 5 ; Huang, Jianxin 6
通讯作者	Huang, Jianxin
发表日期	2022-08-01
DOI	10.23812/j.biol.regul.homeost.agents.20223604.121
发表期刊	JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 影响因子和分区
ISSN	0393-974X
EISSN	1724-6083
卷号	36 期号:4
摘要	Background: Tumor-specific neoantigens are ideal targets for cancer immunotherapy, but few of these have been identified thus far. An analysis strategy was developed for neoantigen in non-coding regions by RNC (ribosome-nascent chain complex)-RNAseq in mouse lung cancer. Methods: In LLC (Lewis lung carcinoma) cell lines, RNC extraction, RNC-RNA extraction, RNC-RNA seq and bioinformatics analysis were carried out. The immunogenicity and specificity were determined by immunizing mice. Results: Twenty neoantigens were identified from non-coding regions. Seventeen long peptides were synthesized successfully and 5/17 of these neoantigens were found to be immunogenic. In tumor transplant models, the non-coding neoantigen vaccines immunization achieved in vivo tumor control in protective and therapeutic settings. Conclusions: In mice, the non-coding neoantigen vaccines can provoke anti-tumour effect against lung cancer. This can form the basis for future study of RNC-RNA-seq, as a method to identify non-coding neoantigens.
关键词	non-coding neoantigens RNC (ribosome-nascent chain complex)-RNA lung cancer immunotherapy mouse
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Fujian Medical University, China[2017XQ1129] ; Fujian Provincial Health Science and Technology Project, China[2020GGB002] ; Guangdong Medical Science and Technology Research Foundation[A2020487]
WOS研究方向	Endocrinology & Metabolism ; Immunology ; Research & Experimental Medicine ; Physiology
WOS类目	Endocrinology & Metabolism ; Immunology ; Medicine, Research & Experimental ; Physiology
WOS记录号	WOS:000891806500001
出版者	BIOLIFE SAS
ESI学科分类	BIOLOGY & BIOCHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：0
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/417063
专题	南方科技大学第一附属医院
作者单位	1.Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Resp & Crit Care Med, Fuzhou 350000, Fujian, Peoples R China 2.Second Mil Med Univ, Shanghai Changhai Hosp, Affiliated Hosp 1, Dept Transfus Med, Shanghai 200000, Peoples R China 3.Xiapu Hosp, Dept Resp, Ningde 352000, Fujian, Peoples R China 4.Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Thorac Surg, Fuzhou 350000, Fujian, Peoples R China 5.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Sch 2,Dept Resp & Crit Care, Shenzhen 518000, Guangdong, Peoples R China 6.Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Clin Lab, Fuzhou, Chin, Myanmar
推荐引用方式 GB/T 7714	Wang, Daxuan,Feng, Ziyang,Lin, Changjian,et al. Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer[J]. JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,2022,36(4).
APA	Wang, Daxuan.,Feng, Ziyang.,Lin, Changjian.,Xie, Tianjian.,Li, Wujin.,...&Huang, Jianxin.(2022).Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer.JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,36(4).
MLA	Wang, Daxuan,et al."Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer".JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 36.4(2022).