Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis

Meng, Shuhui; Wang, Tingting; Zhao, Qianqian; Hu, Qiu; Chen, Yulan; Li, Heng; Liu, Cuilian; Liu, Dongzhou; Hong, Xiaoping

2022-12-01

10.1021/acs.jproteome.2c00532

JOURNAL OF PROTEOME RESEARCH   影响因子和分区

1535-3893

1535-3907

Dermatomyositis and polymyositis (DM/PM) are systemic autoimmune diseases characterized by proximal muscle weakness. The underlying pathogenetic mechanism of this disease remains under-researched. Here, using proteomics analysis, a great overlap of differentially expressed plasma exosomal proteins involved in the complement and coagulation cascade pathway, including FGA, FGB, FGG, C1QB, C1QC, and VWF, was identified in DM/PM patients versus healthy controls. Correlation analysis showed that the expression levels of complement-associated proteins (C1QB and C1QC) correlated positively with CRP, ESR, and platelet count. ROC curve analysis demonstrated that complement and coagulation cascade-associated proteins could be strong predictors for DM/ PM. In addition, we also identified several other proteins that were differentially expressed in DM and PM. The selected candidate proteins were further validated by parallel reaction monitoring (PRM) and enzyme-linked immunosorbent assay (ELISA). Together, our findings indicate that these exosome-derived proteins might participate in microvascular damage in DM/PM through the activation of the complement and coagulation cascade pathway and function as biomarkers for the clinical diagnosis of DM/PM.

dermatomyositis polymyositis exosomes proteomics complement and coagulation cascades

SCI

英语

第一 ; 通讯

[SZXK011] ; [SZSM202111006]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000895914800001

AMER CHEMICAL SOC

BIOLOGY & BIOCHEMISTRY

Web of Science

Southern Univ Sci & Technol, Jinan Univ, Dept Rheumatol & Immunol, Shenzhen Peoples Hosp,Clin Med Coll 2,Affiliated H, Shenzhen 518020, Guangdong, Peoples R China

Meng, Shuhui,Wang, Tingting,Zhao, Qianqian,et al. Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis[J]. JOURNAL OF PROTEOME RESEARCH,2022,22(1).

Meng, Shuhui.,Wang, Tingting.,Zhao, Qianqian.,Hu, Qiu.,Chen, Yulan.,...&Hong, Xiaoping.(2022).Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis.JOURNAL OF PROTEOME RESEARCH,22(1).

Meng, Shuhui,et al."Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis".JOURNAL OF PROTEOME RESEARCH 22.1(2022).