Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation

Riau, Andri K.1,2; Aung, Thet T.3; Setiawan, Melina1; Yang, Liang4; Yam, Gary H. F.1,5; Beuerman, Roger W.3,5,6; Venkatraman, Subbu S.2; Mehta, Jodhbir S.1,2,5,7

2019-09

10.3390/pathogens8030093

Pathogens   影响因子和分区

2076-0817

2076-0817

Bacterial biofilm on medical devices is difficult to eradicate. Many have capitalized the anti-infective capability of silver ions (Ag+) by incorporating nano-silver (nAg) in a biodegradable coating, which is then laid on polymeric medical devices. However, such coating can be subjected to premature dissolution, particularly in harsh diseased tissue microenvironment, leading to rapid nAg clearance. It stands to reason that impregnating nAg directly onto the device, at the surface, is a more ideal solution. We tested this concept for a corneal prosthesis by immobilizing nAg and nano-hydroxyapatite (nHAp) on poly(methyl methacrylate), and tested its biocompatibility with human stromal cells and antimicrobial performance against biofilm-forming pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Three different dual-functionalized substrates-high Ag (referred to as 75:25 HAp:Ag); intermediate Ag (95:5 HAp:Ag); and low Ag (99:1 HAp:Ag) were studied. The 75:25 HAp:Ag was effective in inhibiting biofilm formation, but was cytotoxic. The 95:5 HAp:Ag showed the best selectivity among the three substrates; it prevented biofilm formation of both pathogens and had excellent biocompatibility. The coating was also effective in eliminating non-adherent bacteria in the culture media. However, a 28-day incubation in artificial tear fluid revealed a similar to 40% reduction in Ag+ release, compared to freshly-coated substrates. The reduction affected the inhibition of S. aureus growth, but not the P. aeruginosa. Our findings suggest that Ag+ released from surface-immobilized nAg diminishes over time and becomes less effective in suppressing biofilm formation of Gram-positive bacteria, such as S. aureus. This advocates the coating, more as a protection against perioperative and early postoperative infections, and less as a long-term preventive solution.

silver toxicity cornea biofilm polymer nanoparticles

SCI

英语

其他

SingHealth Foundation[SHF/FG661P/2017]

Microbiology

Microbiology

WOS:000488003600053

MDPI

Web of Science

1.Singapore Eye Res Inst, Tissue Engn & Stem Cell Grp, Singapore 169856, Singapore
2.Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore 639798, Singapore
3.Singapore Eye Res Inst, Antiinfect Res Grp, Singapore 169856, Singapore
4.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
5.Duke Natl Univ, Ophthalmol & Visual Sci ACP, Singapore Med Sch, Singapore 169857, Singapore
6.Duke Natl Univ, SRP Neurosci & Emerging Infect Dis, Singapore Med Sch, Singapore 169857, Singapore
7.Singapore Natl Eye Ctr, Corneal & External Eye Dis Serv, Singapore 168751, Singapore

Riau, Andri K.,Aung, Thet T.,Setiawan, Melina,et al. Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation[J]. Pathogens,2019,8(3).

Riau, Andri K..,Aung, Thet T..,Setiawan, Melina.,Yang, Liang.,Yam, Gary H. F..,...&Mehta, Jodhbir S..(2019).Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation.Pathogens,8(3).

Riau, Andri K.,et al."Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation".Pathogens 8.3(2019).