Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa

Li, Chao1; Ran, Minyuan1,2; Liu, Jianwei1; Wang, Xiaoxiao1,2; Wu, Qingbing1,2; Zhang, Qiang3; Yang, Jing3; Yi, Feng3; Zhang, Heng4; Zhu, Jian-Kang5,6; Zhao, Chunzhao1

2022-12-12

10.3389/fpls.2022.1083438

Frontiers in Plant Science   影响因子和分区

1664-462X

Protochlorophyllide oxidoreductase (POR) plays a key role in catalyzing the light-dependent reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), and thus promotes the transit from etiolated seedlings to green plants. In this study, by exploring ethyl methanesulfonate (EMS)-mediated mutagenesis in Chenopodium quinoa NL-6 variety, we identified a mutant nl6-35 that displays faded green leaf and reduced chlorophyll (Chl) and carotenoid contents. Bulk segregant analysis (BSA) revealed that a mutation in CqPORB gene is genetically associated with the faded green leaf of the nl6-35 mutant. Further study indicates that the nl6-35 mutant exhibits abnormal grana stacks and compromised conversion of Pchlide to Chlide upon illumination, suggesting the important role of CqPORB in producing photoactive Pchlide. Totally three CqPOR isoforms, including CqPORA, CqPORA-like, and CqPORB are identified in NL-6 variety. Transcriptional analysis shows that the expression of all these three CqPOR isoforms is regulated in light- and development-dependent manners, and in mature quinoa plants only CqPORB isoform is predominantly expressed. Subcellular localization analysis indicates that CqPORB is exclusively localized in chloroplast. Together, our study elucidates the important role of CqPORB in the regulation of Chl biosynthesis and chloroplast development in quinoa.

protochlorophyllide oxidoreductase (POR) quinoa (Chenopodium quinoa Willd) protochlorophyllide chlorophyll grana stacks

SCI

英语

其他

Plant Sciences

Plant Sciences

WOS:000901923600001

FRONTIERS MEDIA SA

Web of Science

1.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Bright Agr Dev Grp Co Ltd, Shanghai, Peoples R China
4.Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China
5.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China
6.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen, Peoples R China

Li, Chao,Ran, Minyuan,Liu, Jianwei,et al. Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa[J]. Frontiers in Plant Science,2022,13.

Li, Chao.,Ran, Minyuan.,Liu, Jianwei.,Wang, Xiaoxiao.,Wu, Qingbing.,...&Zhao, Chunzhao.(2022).Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa.Frontiers in Plant Science,13.

Li, Chao,et al."Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa".Frontiers in Plant Science 13(2022).