High-frequency mutation and recombination are responsible for the emergence of novel porcine reproductive and respiratory syndrome virus in northwest China

Zhang, Xun1,2; Li, Yan1; Xiao, Shengzhong1; Yang, Xia1; Chen, Xinkai1; Wu, Peng1; Song, Jiawei1; Ma, Zhenguo1; Cai, Zhuoxuan1; Jiang, Mengmeng1; Zhang, Yanhong1; Yang, Yan1; Zhang, Zhe1; Zhou, Ziheng1; Sheng, Jinliang1; Wang, Heng3,4

2019-11

10.1007/s00705-019-04373-z

ARCHIVES OF VIROLOGY   影响因子和分区

0304-8608

1432-8798

Porcine reproductive and respiratory syndrome (PRRS) is one of the most highly infectious diseases in the pig industry, resulting in enormous economic losses worldwide. In this study, a PRRS virus (PRRSV) strain was isolated from primary porcine alveolar macrophage cells in Xinjiang in northwest China. This new strain was sequenced and designated as XJzx1-2015, and its sequence was then compared to those of other representative PRRSV strains from around the world. Complete genomic characterisation showed that the full-length nucleotide sequence of XJzx1-2015 exhibited low-level similarity to NB/04 (91.6%), JXA1 (90.5%), CH-1a (90.2%), VR-2332 (86.9%), QYYZ (85.7%), and JL580 (82.2%), with the highest similarity to HK13 (91.7%) sequence identity. Nonstructural protein 2 (NSP2) and glycosylated protein (GP) 2 of XJzx1-2015 had deletions of five and two amino acids, respectively, corresponding to strain VR-2332 positions 475-479 and 173-174. Phylogenetic analysis based on complete genome sequences showed that XJzx1-2015 and four other strains from China formed a new subgenotype closely related to other sublineage 8.7 (JXA1-like) strains belonging to the North American genotype. However, phylogenetic analysis based on NSP2 and GP5 showed that XJzx1-2015 clustered with sublineage 8.7 (JXA1-like, CH-1a-like) and lineage 3 (QYYZ-like) strains, respectively. Recombination analysis indicated that XJzx1-2015 is an intersubgenotype recombinant of CH-1a-like and QYYZ-like strains. Overall, our findings demonstrate that XJzx1-2015 is a novel PRRSV strain with a significantly high frequency of mutation and a recombinant between lineage 3 and sublineage 8.7 identified in northwest China. These results provide important insights into PRRSV evolution.

SCI

英语

其他

Xinjiang Bingtuan Science and Technology Development Fund[2017BA044]

Virology

Virology

WOS:000488510500009

SPRINGER WIEN

MICROBIOLOGY

Web of Science

1.Shihezi Univ, Coll Anim Sci & Technol, 2 221 North Fourth Rd, Shihezi 832000, Xinjiang, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
3.South China Agr Univ, Coll Vet Med, Guangzhou 510000, Guangdong, Peoples R China
4.South China Agr Univ, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China

Zhang, Xun,Li, Yan,Xiao, Shengzhong,et al. High-frequency mutation and recombination are responsible for the emergence of novel porcine reproductive and respiratory syndrome virus in northwest China[J]. ARCHIVES OF VIROLOGY,2019,164(11):2725-2733.

Zhang, Xun.,Li, Yan.,Xiao, Shengzhong.,Yang, Xia.,Chen, Xinkai.,...&Wang, Heng.(2019).High-frequency mutation and recombination are responsible for the emergence of novel porcine reproductive and respiratory syndrome virus in northwest China.ARCHIVES OF VIROLOGY,164(11),2725-2733.

Zhang, Xun,et al."High-frequency mutation and recombination are responsible for the emergence of novel porcine reproductive and respiratory syndrome virus in northwest China".ARCHIVES OF VIROLOGY 164.11(2019):2725-2733.