Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis

Zhang Siwei1; Wu Zhen2; Zhou Zhi3; Hu Xuguang2; Li Yousheng1

2019

10.14715/cmb/2019.65.7.13

CELLULAR AND MOLECULAR BIOLOGY   影响因子和分区

0145-5680

1165-158X

This study was aimed to investigate the anti-tumor activity of rubimaillin in vitro, and the mechanism involved. The inhibitory effect of rubimaillin on cell proliferation was determined with MTT assay. Apoptosis was assayed using AV/PI double staining, while the mitochondrial membrane potential of SKOV-3 cells was determined with Rhodamine 123 (Rh123) staining. Western blot assay was used to determine the effect of rubimaillin on the expressions of Bcl-2, Bax, PARP, cleaved PARP, caspase3, cleaved caspase3, and other apoptosis-related proteins in SKOV-3 cells. Rubimaillin inhibited the growth of SKOV-3 cells in a concentration-dependent manner and induced apoptosis of tumor cells through the mitochondrial apoptosis pathway. These results indicate that rubimaillin is a potential anti-ovarian cancer drug.

Rubimaillin Ovarian cancer Mitochondria-dependent apoptosis

SCI

英语

第一 ; 通讯

Funds of Shenzhen Special Specialty Project of Traditional Chinese Medicine in 2018. (Shenzhen Municipal Health Commissiom traditional Chinese medicine [2018])[51]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000489886900013

C M B ASSOC

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Dept Tradit Chinese Med, Clin Med Coll 2,Shenzhen Peoples Hosp,Affiliated, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China
2.Guangdong Pharmaceut Univ, Sch Tradit Chinese Med, 280 Daxuecheng Outer Ring East Rd, Guangzhou 510006, Guangdong, Peoples R China
3.Shenzhen Inst Drug Control, Microbiol Lab, 28 Gaoxin Middle Second Rd, Shenzhen 518057, Guangdong, Peoples R China

Zhang Siwei,Wu Zhen,Zhou Zhi,et al. Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis[J]. CELLULAR AND MOLECULAR BIOLOGY,2019,65(7):72-76.

Zhang Siwei,Wu Zhen,Zhou Zhi,Hu Xuguang,&Li Yousheng.(2019).Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis.CELLULAR AND MOLECULAR BIOLOGY,65(7),72-76.

Zhang Siwei,et al."Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis".CELLULAR AND MOLECULAR BIOLOGY 65.7(2019):72-76.