Cardiotoxicity of forchlorfenuron (CPPU) in zebrafish (Danio rerio) and H9c2 cardiomyocytes

Gong, Guiyi1,2,3; Kam, Hiotong1,2; Tse, Yuchung3; Lee, Simon Mingyuen1,2

2019-11

10.1016/j.chemosphere.2019.06.027

CHEMOSPHERE   影响因子和分区

0045-6535

1879-1298

Forchlorfenuron (CPPU), as a plant growth regulator or herbicide/pesticide, is widely used in agriculture worldwide. It is adopted by most farmers due to its high efficacy for boosting size and improving the quality of fruit. However, CPPU was implicated in, and gained notoriety due to an incident of exploding watermelon that occurred in 2011. Subsequently, the wider community became aware of the potential risks it posed to living organisms and the ecosystem. In this study, we evaluated the effects of CPPU on the survival, cardiac morphology and function, as well as hematopoietic system, of zebrafish (Danio rerio). Notably, CPPU (2.5-12.5 mu g/ml) induced cardiac morphology deformation, cardiac contractile dysfunction and erythrocyte reduction in zebrafish. Consistently, the mRNA expression levels of several cardiac and hematopoietic gene markers (myl7, gata4, mef2c, amhc, vmhc and gata1) were altered by CPPU treatment. In addition, CPPU caused cytotoxicity, cytoskeleton destruction and reduced corresponding proteins (Myl7, Gata4 and Mef2c) expression in H9c2 cardiomyocytes in vitro. Taken together, this study has identified the cardiotoxicity of CPPU in different experimental models and enhanced our understanding on the mechanism underlying the toxicity of CPPU to living organisms. (C) 2019 Elsevier Ltd. All rights reserved.

Forchlorfenuron (CPPU) Zebrafish Cardiotoxicity Hematopoietic toxicity H9c2 cardiomyocytes Cytoskeletons destruction

SCI ; EI

英语

通讯

Research Committee, University of Macau[MYRG2016-00133-ICMSQRCM]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000487567000017

PERGAMON-ELSEVIER SCIENCE LTD

20192607112677

Agriculture ; Gene expression ; Growth kinetics ; Morphology ; Toxicity

Bioengineering and Biology:461 ; Agricultural Equipment and Methods; Vegetation and Pest Control:821 ; Materials Science:951

ENVIRONMENT/ECOLOGY

Web of Science

1.Univ Macau, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
3.Southern Univ Sci & Technol SUSTech, Dept Biol, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen 518055, Peoples R China

Gong, Guiyi,Kam, Hiotong,Tse, Yuchung,et al. Cardiotoxicity of forchlorfenuron (CPPU) in zebrafish (Danio rerio) and H9c2 cardiomyocytes[J]. CHEMOSPHERE,2019,235:153-162.

Gong, Guiyi,Kam, Hiotong,Tse, Yuchung,&Lee, Simon Mingyuen.(2019).Cardiotoxicity of forchlorfenuron (CPPU) in zebrafish (Danio rerio) and H9c2 cardiomyocytes.CHEMOSPHERE,235,153-162.

Gong, Guiyi,et al."Cardiotoxicity of forchlorfenuron (CPPU) in zebrafish (Danio rerio) and H9c2 cardiomyocytes".CHEMOSPHERE 235(2019):153-162.