Active DNA demethylation in plants: 20 years of discovery and beyond

Zhang, Heng1; Gong, Zhizhong2,3; Zhu, Jian-Kang4

2022-12-01

10.1111/jipb.13423

Journal of Integrative Plant Biology   影响因子和分区

1672-9072

1744-7909

Maintaining proper DNA methylation levels in the genome requires active demethylation of DNA. However, removing the methyl group from a modified cytosine is chemically difficult and therefore, the underlying mechanism of demethylation had remained unclear for many years. The discovery of the first eukaryotic DNA demethylase, Arabidopsis thaliana REPRESSOR OF SILENCING 1 (ROS1), led to elucidation of the 5-methylcytosine base excision repair mechanism of active DNA demethylation. In the 20 years since ROS1 was discovered, our understanding of this active DNA demethylation pathway, as well as its regulation and biological functions in plants, has greatly expanded. These exciting developments have laid the groundwork for further dissecting the regulatory mechanisms of active DNA demethylation, with potential applications in epigenome editing to facilitate crop breeding and gene therapy.

abiotic stress response base excision repair DNA demethylation DNA methylation histone modification plant growth and development plant microbe interaction

SCI

英语

通讯

[31922008] ; [XDB27040108] ; [X20200101] ; [2021YFA1300401]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:000905780800002

WILEY

Web of Science

1.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Ctr Excellence Mol Plant Sci, State Key Lab Mol Plant Genet, Shanghai 200032, Peoples R China
2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China
3.Hebei Univ, Inst Life Sci & Green Dev, Sch Life Sci, Baoding 071002, Peoples R China
4.Southern Univ Sci & Technol, Inst Adv Biotechnol, Sch Life Sci, Shenzhen 518055, Peoples R China

Zhang, Heng,Gong, Zhizhong,Zhu, Jian-Kang. Active DNA demethylation in plants: 20 years of discovery and beyond[J]. Journal of Integrative Plant Biology,2022,64(12).

Zhang, Heng,Gong, Zhizhong,&Zhu, Jian-Kang.(2022).Active DNA demethylation in plants: 20 years of discovery and beyond.Journal of Integrative Plant Biology,64(12).

Zhang, Heng,et al."Active DNA demethylation in plants: 20 years of discovery and beyond".Journal of Integrative Plant Biology 64.12(2022).