CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway

Chen, Erbao1,2; He, Yu2; Jiang, Jing3; Yi, Jing1; Zou, Zhilin4; Song, Qiuzi1; Ren, Qingqi1; Lin, Zewei1; Lu, Yi2,5; Liu, Jikui1; Zhang, Jian2,5

2023-01-13

10.1186/s40164-022-00366-y

EXPERIMENTAL HEMATOLOGY & ONCOLOGY   影响因子和分区

2162-3619

BackgroundHepatocellular carcinoma (HCC) is one of the most lethal malignant tumors. Cell division cycle associated 8 (CDCA8) is an important multifactorial regulator in cancers. However, its up and downstream targets and effects in HCC are still unclear.MethodsA comprehensive bioinformatics analysis was performed using The Cancer Genome Atlas dataset (TCGA) to explore novel core oncogenes. We quantified CDCA8 levels in HCC tumors using qRT-PCR. HCC cell's proliferative, migratory, and invasive abilities were detected using a Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2 '-deoxyuridine (EdU) assay, clone formation, and a Transwell assay. An orthotopic tumor model and tail vein model were constructed to determine the effects of CDCA8 inhibition in vivo. The mechanism underlying CDCA8 was investigated using RNA sequencing. The prognostic value of CDCA8 was assessed with immunohistochemical staining of the tissue microarrays.ResultsCDCA8 was identified as a novel oncogene during HCC development. The high expression of CDCA8 was an independent predictor for worse HCC outcomes both in publicly available datasets and in our cohort. We found that CDCA8 knockdown inhibited HCC cell proliferation, colony formation, and migration by suppressing the MEK/ERK pathway in vitro. Moreover, CDCA8 deficiency significantly inhibited tumorigenesis and metastasis. Next-generation sequencing and laboratory validation showed that CDCA8 silencing inhibited the expression of TPM3, NECAP2, and USP13. Furthermore, NA-YA overexpression upregulated the expression of CDCA8. CDCA8 knockdown could attenuate NF-YA-mediated cell invasion in vitro. The expression of NF-YA alone or in combined with CDCA8 were validated as significant independent risk factors for patient survival.ConclusionOur findings revealed that the expression of CDCA8 alone or in combined with NF-YA contributed to cancer progression, and could serve as novel potential therapeutic targets for HCC patients.

Hepatocellular carcinoma Cell division cycle associated 8 NF-YA Cancer metastasis Prognosis

SCI

英语

通讯

Oncology ; Hematology

Oncology ; Hematology

WOS:000912748800001

BMC

2-s2.0-85146338992

Web of Science

1.Peking Univ, Shenzhen Hosp, Dept Hepatobiliary & Pancreat Surg, Shenzhen 518036, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Pathol, Shenzhen, Guangdong, Peoples R China
4.Wenzhou Med Univ, Affiliated Eye Hosp, Dept Ophthalmol, Wenzhou, Zhejiang, Peoples R China
5.Guangdong Prov Key Lab Cell Microenvironm & Dis Re, Shenzhen, Guangdong, Peoples R China

Chen, Erbao,He, Yu,Jiang, Jing,et al. CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway[J]. EXPERIMENTAL HEMATOLOGY & ONCOLOGY,2023,12(1).

Chen, Erbao.,He, Yu.,Jiang, Jing.,Yi, Jing.,Zou, Zhilin.,...&Zhang, Jian.(2023).CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway.EXPERIMENTAL HEMATOLOGY & ONCOLOGY,12(1).

Chen, Erbao,et al."CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway".EXPERIMENTAL HEMATOLOGY & ONCOLOGY 12.1(2023).