Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections

Li, Wenmin1; Wang, Xiaoxiao1; Cui, Wenhao1; Yuan, Leyong2; Hu, Xuejiao1

2023

10.3390/pathogens12010021

PATHOGENS   影响因子和分区

2076-0817

Reliable diagnostics are necessary to identify influenza infections, and coronavirus disease 2019 (COVID-19) highlights the need to develop highly specific and sensitive viral detection methods to distinguish severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory pathogens to prevent their further spread. In this prospective study, 1070 clinical respiratory samples were collected from patients with acute respiratory infections from January 2019 to February 2021 to evaluate the diagnostic performance of a multiplex probe amplification (MPA) assay, designed to screen 18 pathogens, mainly those causing acute respiratory infections. Ninety-six positive samples and twenty negative samples for the 18 respiratory pathogens defined by the MPA assay and reverse transcription polymerase chain reaction (RT-PCR) were further confirmed by reference next-generation sequencing (NGS). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the MPA assay were 95.00%, 93.75%, 98.96% and 75.00%, respectively. Additionally, the co-infection rate for these positive samples were 25% (24/95). The MPA assay demonstrated a highly concordant diagnostic performance with NGS in the diagnosis of 18 respiratory pathogens and might play an important role in clinical respiratory pathogen diagnosis.

clinical evaluation SARS-CoV-2 multiplex probe amplification technique respiratory pathogens diagnosis next-generation sequencing

SCI

英语

通讯

Microbiology

Microbiology

WOS:000915636100001

MDPI

Web of Science

1.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Div Lab Med, Guangzhou 510080, Peoples R China
2.Southern Univ Sci & Technol Hosp, Dept Clin Lab, Shenzhen 518055, Peoples R China

Li, Wenmin,Wang, Xiaoxiao,Cui, Wenhao,et al. Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections[J]. PATHOGENS,2023,12(1).

Li, Wenmin,Wang, Xiaoxiao,Cui, Wenhao,Yuan, Leyong,&Hu, Xuejiao.(2023).Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections.PATHOGENS,12(1).

Li, Wenmin,et al."Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections".PATHOGENS 12.1(2023).