Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon

Ye，Quanhui1,2; Liang，Chengyue1; Chen，Xunwen2,3; Fang，Tingting1; Wang，Yun1; Wang，Hui1

2019-12-01

10.1016/j.jes.2019.04.027

Journal of Environmental Sciences   影响因子和分区

1001-0742

1878-7320

Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons (PAHs) is crucial to developing strategies for PAHs bioremediation. In this study, the linkage between the type of PAHs and microbial community structure was fully investigated through 16S rRNA gene sequencing on four PAH-degrading cultures. Putative degradation products were also detected. Our results indicated that naphthalene (Nap)/2-methylnaphthalene (2-Nap), phenanthrene (Phe) and anthracene (Ant) sculpted different microbial communities. Among them, Nap and 2-Nap selected for similar degrading bacteria (i.e., Alicycliphilus and Thauera) and methanogens (Methanomethylovorans and Methanobacterium). Nap and 2-Nap were probably activated via carboxylation, producing 2-naphthoic acid. In contrast, Phe and Ant shaped different bacterial and archaeal communities, with Arcobacter and Acinetobacter being Phe-degraders and Thiobacillus Ant-degrader. Methanogenic archaea Methanobacterium and Methanomethylovorans predominated Phe-degrading and Ant-degrading culture, respectively. These findings can improve our understanding of natural PAHs attenuation and provide some guidance for PAHs bioremediation in methanogenic environment.

Carboxylation Degradation products Degrading bacteria Microbial community structure Polycyclic aromatic hydrocarbons

SCI ; EI

英语

其他

National Water Pollution Control and Treatment Science and Technology Major Project[2017ZX07202002]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000499949600009

SCIENCE PRESS

20192206994167

Anthracene ; Aromatization ; Bacteria ; Bioremediation ; Biotechnology ; Carboxylation ; Degradation ; Mineral oils ; Naphthalene ; Polycyclic aromatic hydrocarbons ; RNA

Environmental Impact and Protection:454.2 ; Biological Materials and Tissue Engineering:461.2 ; Biotechnology:461.8 ; Petroleum Products:513.3 ; Chemical Reactions:802.2 ; Organic Compounds:804.1

2-s2.0-85066268068

Scopus

1.State Key Joint Laboratory on Environment Simulation and Pollution ControlSchool of EnvironmentTsinghua University,Beijing,100084,China
2.Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution ControlSchool of Environmental Science and EngineeringSouthern University of Science and Technology of China,Shenzhen,518055,China
3.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution ControlSchool of Environmental Science and EngineeringSouthern University of Science and Technology,Shenzhen,518055,China

Ye，Quanhui,Liang，Chengyue,Chen，Xunwen,et al. Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon[J]. Journal of Environmental Sciences,2019,86:97-106.

Ye，Quanhui,Liang，Chengyue,Chen，Xunwen,Fang，Tingting,Wang，Yun,&Wang，Hui.(2019).Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon.Journal of Environmental Sciences,86,97-106.

Ye，Quanhui,et al."Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon".Journal of Environmental Sciences 86(2019):97-106.