Fabrication and Biomedical Applications of "polymer-Like" Nucleic Acids Enzymatically Produced by Rolling Circle Amplification

Li，Jing; Lin，Li; Yu，Jiantao; Zhai，Shiyao; Liu，Guoyuan; Tian，Leilei

2019-10-21

10.1021/acsabm.9b00622

ACS Applied Bio Materials   影响因子和分区

25766422

2576-6422

Rolling circle amplification (RCA) is an isothermal enzymatic amplification reaction, which is used to massively produce periodic long-single-stranded DNA/RNA with predesigned sequences and functions. The RCA technique not only shows unparalleled advantages in constructing functional DNA assemblies; and the RCA products can also serve as high-performance scaffolds to interact with or accommodate foreign moieties, i.e., therapeutic nucleic acids, proteins, lipids, cationic polymers, and metal nanoparticles (NPs), etc. to fabricate multifunctional and bioresponsive materials with various sizes and morphologies. The RCA product is also a kind of high-molecular-weight polyanion with strong inter/intra-molecular interactions, which provides great opportunities for regulating the condensation states of DNA nanomedicines and bulk materials. Based on all these good properties, the RCA-based materials have shown more and more practical potentials in biomedical fields. In this review, we summarize the recent development of RCA-based origami structures, NPs, hydrogels, and metallization, for which the fabrication methods, their biomedical applications, and future prospects are carefully discussed.

biosensing and biomedical application DNA condensation DNA hydrogel DNA metallization DNA origami nanomedicine rolling circle amplification

EI

英语

第一 ; 通讯

Shenzhen Fundamental Research and Discipline Layout project[JCYJ20160226193029593] ; National Natural Science Foundation of China[51503096] ; Guangdong Innovative and Entrepreneurial Research Team Program[2016ZT06G587]

American Chemical Society

20194107496587

Condensation ; DNA ; DNA sequences ; Fabrication ; Hydrogels ; Medical applications ; Medical nanotechnology ; Metal nanoparticles ; Metallizing ; Rolling ; Scaffolds ; Scaffolds (biology)

Construction Equipment:405.1 ; Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Metal Plating:539.3 ; Nanotechnology:761 ; Chemical Operations:802.3 ; Chemical Products Generally:804 ; Polymeric Materials:815.1

2-s2.0-85072846325

Scopus

Department of Materials Science and EngineeringSouthern University of Science and Technology,Shenzhen, Guangdong,1088 Xueyuan Boulevard, Nanshan District,518055,China

Li，Jing,Lin，Li,Yu，Jiantao,et al. Fabrication and Biomedical Applications of "polymer-Like" Nucleic Acids Enzymatically Produced by Rolling Circle Amplification[J]. ACS Applied Bio Materials,2019,2(10):4106-4120.

Li，Jing,Lin，Li,Yu，Jiantao,Zhai，Shiyao,Liu，Guoyuan,&Tian，Leilei.(2019).Fabrication and Biomedical Applications of "polymer-Like" Nucleic Acids Enzymatically Produced by Rolling Circle Amplification.ACS Applied Bio Materials,2(10),4106-4120.

Li，Jing,et al."Fabrication and Biomedical Applications of "polymer-Like" Nucleic Acids Enzymatically Produced by Rolling Circle Amplification".ACS Applied Bio Materials 2.10(2019):4106-4120.