| 题名 | Structural insight into RNA synthesis by influenza D polymerase |
| 作者 | |
| 通讯作者 | Gao，George F. |
| 发表日期 | 2019-10-01
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| DOI | |
| 发表期刊 | |
| ISSN | 2058-5276
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| EISSN | 2058-5276
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| 卷号 | 4期号:10页码:1750-1759 |
| 摘要 | The influenza virus polymerase uses capped RNA primers to initiate transcription, and a combination of terminal and internal de novo initiations for the two-step replication process by binding the conserved viral genomic RNA (vRNA) or complementary RNA (cRNA) promoter. Here, we determined the apo and promoter-bound influenza D polymerase structures using cryo-electron microscopy and found the polymerase has an evolutionarily conserved stable core structure with inherently flexible peripheral domains. Strikingly, two conformations (mode A and B) of the vRNA promoter were observed where the 3ʹ-vRNA end can bind at two different sites, whereas the cRNA promoter only binds in the mode B conformation. Functional studies confirmed the critical role of the mode B conformation for vRNA synthesis via the intermediate cRNA but not for cRNA production, which is mainly regulated by the mode A conformation. Both conformations participate in the regulation of the transcription process. This work advances our understanding of the regulatory mechanisms for the synthesis of different RNA species by influenza virus polymerase and opens new opportunities for antiviral drug design. |
| 相关链接 | [Scopus记录] |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 其他
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| 资助项目 | Youth Innovation Promotion Association CAS[2015078]
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| WOS研究方向 | Microbiology
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| WOS类目 | Microbiology
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| WOS记录号 | WOS:000487286800020
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| 出版者 | |
| Scopus记录号 | 2-s2.0-85068108611
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| 来源库 | Scopus
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| 引用统计 |
被引频次[WOS]：53
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/43862 |
| 专题 | 生命科学学院_生物系 生命科学学院 |
| 作者单位 | 1.CAS Key Laboratory of Pathogenic Microbiology and ImmunologyInstitute of MicrobiologyChinese Academy of Sciences,Beijing,China 2.Institute of Physical Science and Information TechnologyAnhui University,Hefei,China 3.Savaid Medical SchoolUniversity of Chinese Academy of Sciences,Beijing,China 4.Research Network of Immunity and HealthBeijing Institute of Life SciencesChinese Academy of Sciences,Beijing,China 5.National Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences,Beijing,China 6.Department of BiologySouthern University of Science and Technology,Shenzhen,China 7.CAS Key Laboratory of Microbial Physiological and Metabolic EngineeringInstitute of MicrobiologyChinese Academy of Sciences,Beijing,China 8.Shenzhen Key Laboratory of Pathogen and ImmunityShenzhen Third People’s Hospital,Shenzhen,China 9.CAS Center for Influenza Research and Early-warningCAS-TWAS Center of Excellence for Emerging Infectious DiseaseChinese Academy of Sciences,Beijing,China 10.MOH Key Laboratory of Systems Biology of PathogensInstitute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China 11.National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention,Beijing,China 12.College of Basic MedicineJilin University,Changchun,China 13.Chongqing Key Laboratory of Neurodegenerative DiseasesChongqing General HospitalUniversity of Chinese Academy of Sciences,Chongqing,China |
| 推荐引用方式 GB/T 7714 |
Peng，Qi,Liu，Yuqian,Peng，Ruchao,et al. Structural insight into RNA synthesis by influenza D polymerase[J]. Nature Microbiology,2019,4(10):1750-1759.
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| APA |
Peng，Qi.,Liu，Yuqian.,Peng，Ruchao.,Wang，Min.,Yang，Wei.,...&Shi，Yi.(2019).Structural insight into RNA synthesis by influenza D polymerase.Nature Microbiology,4(10),1750-1759.
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| MLA |
Peng，Qi,et al."Structural insight into RNA synthesis by influenza D polymerase".Nature Microbiology 4.10(2019):1750-1759.
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| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | 操作 | |
| Structural insight i（3705KB） | -- | -- | 限制开放 | -- | ||
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