Pleiotropic effects of c-di-GMP content in Pseudomonas syringae

Wang，Tingting1; Cai，Zhao2; Shao，Xiaolong3; Zhang，Weitong1; Xie，Yingpeng1; Zhang，Yingchao3; Hua，Canfeng1; Schuster，Stephan C.2,5; Yang，Liang2,4; Deng，Xin1

2019-05-01

10.1128/AEM.00152-19

APPLIED AND ENVIRONMENTAL MICROBIOLOGY   影响因子和分区

0099-2240

1098-5336

Although the ubiquitous bacterial secondary messenger cyclic diguanylate (c-di-GMP) has important cellular functions in a wide range of bacteria, its function in the model plant pathogen Pseudomonas syringae remains largely elusive. To this end, we overexpressed Escherichia coli diguanylate cyclase (YedQ) and phosphodiesterase (YhjH) in P. syringae, resulting in high and low in vivo levels of c-di- GMP, respectively. Via genome-wide RNA sequencing of these two strains, we found that c-di-GMP regulates (i) fliN, fliE, and flhA, which are associated with flagellar assembly; (ii) alg8 and alg44, which are related to the exopolysaccharide biosynthesis pathway; (iii) pvdE, pvdP, and pvsA, which are associated with the siderophore biosynthesis pathway; and (iv) sodA, which encodes a superoxide dismutase. In particular, we identified three promoters that are sensitive to elevated levels of c-di-GMP and inserted them into luciferase-based reporters that respond effectively to the c-di-GMP levels in P. syringae; these promoters could be useful in the measurement of in vivo levels of c-di-GMP in real time. Further phenotypic assays validated the RNA sequencing (RNA-seq) results and confirmed the effect on c-di-GMP-associated pathways, such as repressing the type III secretion system (T3SS) and motility while inducing biofilm production, siderophore production, and oxidative stress resistance. Taken together, these results demonstrate that c-di-GMP regulates the virulence and stress response in P. syringae, which suggests that tuning its level could be a new strategy to protect plants from attacks by this pathogen.

C-di-GMP Pleiotropic effects Pseudomonas syringae T3SS

SCI ; EI

英语

其他

National Natural Science Foundation of China[31670127] ; National Natural Science Foundation of China[31870116]

WOS:000466715500006

American Society for Microbiology

20192006930798

Biochemistry ; Biosynthesis ; Enzymes ; Escherichia coli ; Molecular biology ; RNA

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Computer Programming Languages:723.1.1 ; Biochemistry:801.2

BIOLOGY & BIOCHEMISTRY

2-s2.0-85065569491

Scopus

1.Department of Biomedical SciencesCity University of Hong Kong,Kowloon Tong,Hong Kong
2.Singapore Centre for Environmental Life Sciences Engineering (SCELSE)Nanyang Technological University,Singapore
3.Key Laboratory of Molecular Microbiology and Technology Ministry of EducationTEDA Institute of Biological Sciences and BiotechnologyNankai University,Tianjin,China
4.School of MedicineSouthern University of Science and Technology (SUSTec),Shenzhen, Guangdong,China
5.School of Biological SciencesNanyang Technological University,Singapore

Wang，Tingting,Cai，Zhao,Shao，Xiaolong,et al. Pleiotropic effects of c-di-GMP content in Pseudomonas syringae[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(10).

Wang，Tingting.,Cai，Zhao.,Shao，Xiaolong.,Zhang，Weitong.,Xie，Yingpeng.,...&Deng，Xin.(2019).Pleiotropic effects of c-di-GMP content in Pseudomonas syringae.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(10).

Wang，Tingting,et al."Pleiotropic effects of c-di-GMP content in Pseudomonas syringae".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.10(2019).