Identification of biomarkers in macrophages of atherosclerosis by microarray analysis

Huang，He Ming1; Jiang，Xin1; Hao，Meng Lei2; Shan，Meng Jie3; Qiu，Yong4; Hu，Gai Feng5; Wang，Quan5; Yu，Zi Qing6; Meng，Ling Bing7; Zou，Yun Yun8

2019-05-01

10.1186/s12944-019-1056-x

Lipids in Health and Disease   影响因子和分区

1476-511X

1476-511X

Background: Atherosclerotic cardiovascular disease (ASCVD) refers to a series of diseases caused by atherosclerosis (AS). It is one of the most important causes of death worldwide. According to the inflammatory response theory, macrophages play a critical role in AS. However, the potential targets associated with macrophages in the development of AS are still obscure. This study aimed to use bioinformatics tools for screening and identifying molecular targets in AS macrophages. Methods: Two expression profiling datasets (GSE7074 and GSE9874) were obtained from the Gene Expression Omnibus dataset, and differentially expressed genes (DEGs) between non-AS macrophages and AS macrophages were identified. Functional annotation of the DEGs was performed by analyzing the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. STRING and Cytoscape were employed for constructing a protein-protein interaction network and analyzing hub genes. Results: A total of 98 DEGs were distinguished between non-AS macrophages and AS macrophages. The functional variations in DEGs were mainly enriched in response to hypoxia, respiratory gaseous exchange, protein binding, and intracellular, ciliary tip, early endosome membrane, and Lys63-specific deubiquitinase activities. Three genes were identified as hub genes, including KDELR3, CD55, and DYNC2H1. Conclusion: Hub genes and DEGs identified by using microarray techniques can be used as diagnostic and therapeutic biomarkers for AS.

Atherosclerosis Atherosclerotic cardiovascular disease Bioinformatics Hub genes Macrophages

SCI

英语

第一

WOS:000466557400002

2-s2.0-85065301843

Scopus

1.Geriatric DepartmentShenzhen People's HospitalSecond Clinical Medical College of Jinan UniversityFirst Affiliated Hospital of Southern University of Science and Technology,Shenzhen,518020,China
2.Department of Geriatric MedicineQinghai University,Xining,810016,China
3.MOH Key Laboratory of Systems Biology of PathogensInstitute of Pathogen BiologyChinese Academy of Medical SciencesPeking Union Medical College,Beijing,100730,China
4.Anesthesiology DepartmentBeijing HospitalNational Center of Gerontology,Beijing,No.1 Dahua Road, Dong Dan,100730,China
5.Department of CardiologyBeijing HospitalNational Center of Gerontology,Beijing,No.1 Dahua Road, Dong Dan,100730,China
6.Pneumology DepartmentBeijing HospitalNational Center of Gerontology,Beijing,No.1 Dahua Road, Dong Dan,100730,China
7.Neurology DepartmentBeijing HospitalNational Center of Gerontology,Beijing,No.1 Dahua Road, Dong Dan,100730,China
8.Fifth Ward of Ophthalmology DepartmentShenzhen Eye Hospital,Shenzhen,518040,China

Huang，He Ming,Jiang，Xin,Hao，Meng Lei,et al. Identification of biomarkers in macrophages of atherosclerosis by microarray analysis[J]. Lipids in Health and Disease,2019,18(1).

Huang，He Ming.,Jiang，Xin.,Hao，Meng Lei.,Shan，Meng Jie.,Qiu，Yong.,...&Zou，Yun Yun.(2019).Identification of biomarkers in macrophages of atherosclerosis by microarray analysis.Lipids in Health and Disease,18(1).

Huang，He Ming,et al."Identification of biomarkers in macrophages of atherosclerosis by microarray analysis".Lipids in Health and Disease 18.1(2019).