CRGD functionalized 2,1,3-benzothiadiazole (BTD)-containing two-photon absorbing red-emitter-conjugated amphiphilic poly(ethylene glycol)-: Block -poly(ϵ-caprolactone) for targeted bioimaging

Wu，Shanshan1; Su，Fengyu2; Magee，Hansa Y.3; Meldrum，Deirdre R.4; Tian，Yanqing5

2019

10.1039/c9ra06694b

RSC Advances   影响因子和分区

20462069

2046-2069

A two-photon absorbing (2PA) red emitter group was chemically conjugated onto amphiphilic poly(ethylene glycol)-block-poly(ϵ-caprolactone) (PEG-b-PCL) copolymers, and further grafted with cyclo(Arg-Gly-Asp) (cRGD) peptide to form micelle 1. Micelle 1 with cRGD targeting groups were used for targeted bioimaging. For comparison, micelle 2 without the cRGD targeting groups were also prepared and investigated. The micelles were characterized using dynamic light scattering (DLS), showing average diameters of around 77 nm. The cRGD targeting group is known to bind specifically with αβ integrin in cancer cells. In this study, αβ integrin overexpressed human glioblastoma U87MG cell line and αβ integrin deficient human cervical cancer HeLa cell line were chosen. Results showed that the cRGD targeting group enhanced the cellular uptake efficiency of the micelles significantly in αβ integrin rich U87MG cells. Higher temperature (37 °C versus 4 °C) and calcium ions (with 3 M calcium chloride in the cell culture medium versus no addition of calcium ions) enhanced the cellular uptake efficiency, suggesting that the uptake of the micelles is through the endocytosis pathway in cells. A 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay was used to evaluate the cytotoxicity of the micelles and no significant cytotoxicity was observed. The BTD-containing two-photon absorbing emitter in the micelles showed a two-photon absorbing cross-section of 236 GM (1 GM = 1 × 10 cm s per photonper molecule) at 820 nm, which is among the highest values reported for red 2PA emitters. Because of the two-photon absorbing characteristics, micelle 1 was successfully used for two-photon fluorescence imaging targeted to U87MG cells under a two-photon fluorescence microscope. This study is the first report regarding the targeted imaging of a specific cancer cell line (herein, U87MG) using the BTD-conjugated-fluorophore-containing block copolymers.

SCI ; EI

英语

其他

Guangdong Industry Polytechnic[KJ2019-003]

Chemistry

Chemistry, Multidisciplinary

WOS:000494684600017

ROYAL SOC CHEMISTRY

20194507636681

Block copolymers ; Calcium chloride ; Cell culture ; Diseases ; Dynamic light scattering ; Efficiency ; Ethylene glycol ; Fluorescence imaging ; Ions ; Lanthanum compounds ; Molecular biology ; Photons ; Polyethylene glycols ; Polyethylene oxides ; Polyols

Biology:461.9 ; Light/Optics:741.1 ; Colloid Chemistry:801.3 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Polymeric Materials:815.1 ; Organic Polymers:815.1.1 ; Production Engineering:913.1 ; Atomic and Molecular Physics:931.3

2-s2.0-85074449374

Scopus

1.Guangdong Industry PolytechnicFoshan Municipality Anti-counterfeiting Engineering Research Center,Guangzhou Guangdong,510300,China
2.Academy for Advanced Interdisciplinary StudiesSouthern University of Science and Technology,Shenzhen Guangdong,518055,China
3.Knowledge EnterpriseArizona State University,Tempe,85287-5001,United States
4.Center for Biosignatures Discovery AutomationBiodesign InstituteArizona State University,Tempe,85287-5001,United States
5.Department of Materials Science and EngineeringSouthern University of Science and Technology,Shenzhen Guangdong,518055,China

Wu，Shanshan,Su，Fengyu,Magee，Hansa Y.,et al. CRGD functionalized 2,1,3-benzothiadiazole (BTD)-containing two-photon absorbing red-emitter-conjugated amphiphilic poly(ethylene glycol)-: Block -poly(ϵ-caprolactone) for targeted bioimaging[J]. RSC Advances,2019,9(59):34235-34243.

Wu，Shanshan,Su，Fengyu,Magee，Hansa Y.,Meldrum，Deirdre R.,&Tian，Yanqing.(2019).CRGD functionalized 2,1,3-benzothiadiazole (BTD)-containing two-photon absorbing red-emitter-conjugated amphiphilic poly(ethylene glycol)-: Block -poly(ϵ-caprolactone) for targeted bioimaging.RSC Advances,9(59),34235-34243.

Wu，Shanshan,et al."CRGD functionalized 2,1,3-benzothiadiazole (BTD)-containing two-photon absorbing red-emitter-conjugated amphiphilic poly(ethylene glycol)-: Block -poly(ϵ-caprolactone) for targeted bioimaging".RSC Advances 9.59(2019):34235-34243.