Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy

Chen，Lichan1; Wei，Xiafei2; Gu，Dayong1; Xu，Yong1; Zhou，Hongzhong1

2023-02-28

10.1016/j.canlet.2022.216048

CANCER LETTERS   影响因子和分区

0304-3835

1872-7980

Liver cancer and disease are among the most socially challenging global health concerns. Although organ transplantation, surgical resection and anticancer drugs are the main methods for the treatment of liver cancer, there are still no proven cures owing to the lack of donor livers and tumor heterogeneity. Recently, advances in tumor organoid technology have attracted considerable attention as they can simulate the spatial constructs and pathophysiological characteristics of tumorigenesis and metastasis in a more realistic manner. Organoids may further contribute to the development of tailored therapies. Combining organoids with other emerging techniques, such as CRISPR-HOT, organ-on-a-chip, and 3D bioprinting, may further develop organoids and address their bottlenecks to create more practical models that generalize different tissue or organ interactions in tumor progression. In this review, we summarize the various methods in which liver organoids may be generated and describe their biological and clinical applications, present challenges, and prospects for their integration with emerging technologies. These rapidly developing liver organoids may become the focus of in vitro clinical model development and therapeutic research for liver diseases in the near future.

3D bioprinting CRISPR-HOT Liver cancer Organ-on-a-chip Organoid

SCI

英语

其他

National Natural Science Foundation of China[82203870] ; Shenzhen Science and Technology Foundation["ZDSYS20210623092001003","JCYJ20210324103 407018","JCYJ20220530163206015","GJHZ20200731095604013","JSGG2 0200807171602031","201906133000069","SGLH20180625171602058"] ; Guangdong Basic and Applied Basic Research Foundation[2021A1515110931] ; Guangdong Science and Technology Foundation["2021A1515220084","2020B1111160001"] ; Open Project of Key Laboratory of Tropical Disease Control of the Ministry of Education (Sun Yat-sen University)[2019kfkt06]

Oncology

Oncology

WOS:000997434700001

ELSEVIER IRELAND LTD

CLINICAL MEDICINE

2-s2.0-85146454165

Scopus

1.Department of Laboratory Medicine,Inst Translat Med,The First Affiliated Hospital of Shenzhen University,Shenzhen Second People's Hospital,Guangzhou Medical University,Guangzhou,China
2.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,China

Chen，Lichan,Wei，Xiafei,Gu，Dayong,et al. Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy[J]. CANCER LETTERS,2023,555.

Chen，Lichan,Wei，Xiafei,Gu，Dayong,Xu，Yong,&Zhou，Hongzhong.(2023).Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy.CANCER LETTERS,555.

Chen，Lichan,et al."Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy".CANCER LETTERS 555(2023).