Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation

Sun, Mengge1,2,3; Hu, Liqiu1; Wang, Shang1; Huang, Tongling2; Zhang, Minyi2; Yang, Meng2; Zhen, Wanxin1; Yang, Dazhi1; Lu, William2,3; Guan, Min2; Peng, Songlin1

2019-11-22

10.1002/jbmr.3892

JOURNAL OF BONE AND MINERAL RESEARCH   影响因子和分区

0884-0431

1523-4681

Circulating microRNAs (miRNAs) play important roles in regulating gene expression and have been reported to be involved in various metabolic diseases, including osteoporosis. Although the transcriptional regulation of osteoblast differentiation has been well characterized, the role of circulating miRNAs in this process is poorly understood. Here we discovered that the level of circulating miR-19b was significantly lower in osteoporotic patients with vertebral compression fractures than that of healthy controls. The expression level of miR-19b was increased during osteoblastic differentiation of human mesenchymal stem cells (hMSCs) and MC3T3-E1 cells, and transfection with synthetic miR-19b could promote osteoblastic differentiation of hMSCs and MC3T3-E1 cells. PTEN (phosphatase and tensin homolog deleted from chromosome 10) was found to be directly repressed by miR-19b, with a concomitant increase in Runx2 expression and increased phosphorylation of AKT (protein kinase B, PKB). The expression level of circulating miR-19b in aged ovariectomized mice was significantly lower than in young mice. Moreover, the osteoporotic bone phenotype in aged ovariectomized mice was alleviated by the injection of chemically modified miR-19b (agomiR-19b). Taken together, our results show that circulating miR-19b plays an important role in enhancing osteoblastogenesis, possibly through regulation of the PTEN/pAKT/Runx2 pathway, and may be a useful therapeutic target in bone loss disorders, such as osteoporosis. (c) 2019 American Society for Bone and Mineral Research.

MICRORNA OSTEOGENESIS OSTEOPOROSIS SCREENING

SCI

英语

第一 ; 通讯

Guangdong Science and Technology Department Project[2016A050503008]

Endocrinology & Metabolism

Endocrinology & Metabolism

WOS:000497903400001

WILEY

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Spine Surg,Affiliated Hosp 1,Coll Med 2, Shenzhen, Guangdong, Peoples R China
2.Chinese Acad Sci, Ctr Human Tissues & Organs Degenerat, Shenzhen Inst Adv Technol, Shenzhen, Guangdong, Peoples R China
3.Univ Hong Kong, Dept Orthopaed & Traumatol, Hong Kong, Peoples R China

Sun, Mengge,Hu, Liqiu,Wang, Shang,et al. Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation[J]. JOURNAL OF BONE AND MINERAL RESEARCH,2019,35(2):306-316.

Sun, Mengge.,Hu, Liqiu.,Wang, Shang.,Huang, Tongling.,Zhang, Minyi.,...&Peng, Songlin.(2019).Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation.JOURNAL OF BONE AND MINERAL RESEARCH,35(2),306-316.

Sun, Mengge,et al."Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation".JOURNAL OF BONE AND MINERAL RESEARCH 35.2(2019):306-316.