COX7A1 suppresses the viability of human non-small cell lung cancer cells via regulating autophagy

Zhao, Lei1,2; Chen, Xin3; Feng, Yetong4; Wang, Guangsuo5; Nawaz, Imran5,6; Hu, Lifu6; Liu, Pengfei1,2

2019-10-30

10.1002/cam4.2659

Cancer Medicine   影响因子和分区

2045-7634

COX7A1 is a subunit of cytochrome c oxidase, and plays an important role in the super-assembly that integrates peripherally into multi-unit heteromeric complexes in the mitochondrial respiratory chain. In recent years, some researchers have identified that COX7A1 is implicated in human cancer cell metabolism and therapy. In this study, we mainly explored the effect of COX7A1 on the cell viability of lung cancer cells. COX7A1 overexpression was induced by vector transfection in NCI-H838 cells. Cell proliferation, colony formation and cell apoptosis were evaluated in different groups. In addition, autophagy was analyzed by detecting the expression level of p62 and LC3, as well as the tandem mRFP-GFP-LC3 reporter assay respectively. Our results indicated that the overexpression of COX7A1 suppressed cell proliferation and colony formation ability, and promoted cell apoptosis in human non-small cell lung cancer cells. Besides, the overexpression of COX7A1 blocked autophagic flux and resulted in the accumulation of autophagosome via downregulation of PGC-1 alpha and upregulation of NOX2. Further analysis showed that the effect of COX7A1 overexpression on cell viability was partly dependent of the inhibition of autophagy. Herein, we identified that COX7A1 holds a key position in regulating the development and progression of lung cancer by affecting autophagy. Although the crosstalk among COX7A1, PGC-1 alpha and NOX2 needs further investigation, our study provides a novel insight into the therapeutic action of COX7A1 against human non-small cell lung cancer.

autophagy cell viability non-small cell lung cancer NOX2 PGC-1 alpha

SCI

英语

第一 ; 通讯

Science and Technology Planning Project of Guangdong Province[2016ZC0244]

Oncology

Oncology

WOS:000493162400001

WILEY

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Jinan Univ,Dept Anesthesiol,Clin Med Coll 2, Shenzhen 518020, Peoples R China
2.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou, Guangdong, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Clin Med Coll 2, Jinan Univ,Shenzhen Peoples Hosp,Dept Lab Med, Shenzhen, Peoples R China
4.Univ Arizona, Coll Med, Dept Med, Tucson, AZ USA
5.Southern Univ Sci & Technol, Affiliated Hosp 1, Dept Thorac Surg, Jinan Univ,Shenzhen Peoples Hosp,Clin Med Coll 2, Shenzhen, Peoples R China
6.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

Zhao, Lei,Chen, Xin,Feng, Yetong,et al. COX7A1 suppresses the viability of human non-small cell lung cancer cells via regulating autophagy[J]. Cancer Medicine,2019,8(18):7762-7773.

Zhao, Lei.,Chen, Xin.,Feng, Yetong.,Wang, Guangsuo.,Nawaz, Imran.,...&Liu, Pengfei.(2019).COX7A1 suppresses the viability of human non-small cell lung cancer cells via regulating autophagy.Cancer Medicine,8(18),7762-7773.

Zhao, Lei,et al."COX7A1 suppresses the viability of human non-small cell lung cancer cells via regulating autophagy".Cancer Medicine 8.18(2019):7762-7773.