Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device

Ouyang，Dongfang1; Li，Yonghua2; He，Wenqi3; Lin，Weicong3; Hu，Lina4; Wang，Chen5; Xu，Liangcheng6; Park，Jaewon3,7; You，Lidan1,6

2019-11-01

10.1063/1.5112050

Biomicrofluidics   影响因子和分区

1932-1058

Multiple myeloma (MM), the disorder of plasma cells, is the second most common type of hematological cancer and is responsible for approximately 20% of deaths from hematological malignancies. The current gold standard for MM diagnosis includes invasive bone marrow aspiration. However, it lacks the sensitivity to detect minimal residual disease, and the nonuniform distribution of clonal plasma cells (CPCs) within bone marrow also often results in inaccurate reporting. Serum and urine assessment of monoclonal proteins, such as Kappa light chains, is another commonly used approach for MM diagnosis. Although it is noninvasive, the level of paraprotein elevation is still too low for detecting minimal residual disease and nonsecretive MM. Circulating CPCs (cCPCs) have been reported to be present in the peripheral blood of MM patients, and high levels of cCPCs were shown to correlate with poor survival. This suggests a potential noninvasive approach for MM disease progress monitoring and prognosis. In this study, we developed a mechanical property-based microfluidic platform to capture cCPCs. Using human myeloma cancer cell lines spiked in healthy donor blood, the microfluidic platform demonstrates high enrichment ratio (>500) and sufficient capture efficiency (40%-55%). Patient samples were also assessed to investigate the diagnostic potential of cCPCs for MM by correlating with the levels of Kappa light chains in patients.

EI ; SCI

英语

通讯

Southern University of Science and Technology[Y01231004] ; [06465-14] ; Guangzhou Municipal Science and Technology Project[201804010284] ; [JCYJ20160331115633182] ; Science and Technology Planning Project of Shenzhen Municipality[] ; Natural Science Foundation of Guangdong Province[2018A0303130042]

Biochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics

Biochemical Research Methods ; Biophysics ; Nanoscience & Nanotechnology ; Physics, Fluids & Plasmas

WOS:000505984000018

American Institute of Physics Inc.

20194807732649

Biomechanics ; Blood ; Bone ; Cell culture ; Cells ; Diseases ; Microfluidics

Bioengineering and Biology:461 ; Microfluidics:632.5.1

2-s2.0-85075380240

Scopus

1.Department of Mechanical and Industrial Engineering,University of Toronto,Toronto,M5S 3G8,Canada
2.Department of Hematology,General Hospital of Southern Theater Command,PLA,Guangzhou,510010,China
3.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.Department of Hematology,Shenzhen People's Hospital,Shenzhen,518020,China
5.Pathology and Laboratory Medicine,Mount Sinai Hospital,Toronto,M5G 1X5,Canada
6.Institute of Biomaterials and Biomedical Engineering,University of Toronto,Toronto,M5S 3G9,Canada
7.Department of Electrical and Electronic Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Ouyang，Dongfang,Li，Yonghua,He，Wenqi,et al. Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device[J]. Biomicrofluidics,2019,13(6).

Ouyang，Dongfang.,Li，Yonghua.,He，Wenqi.,Lin，Weicong.,Hu，Lina.,...&You，Lidan.(2019).Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device.Biomicrofluidics,13(6).

Ouyang，Dongfang,et al."Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device".Biomicrofluidics 13.6(2019).