南方科技大学知识苑(SUSTech KC): Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants

题名	Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants
作者	Bissoyi, Akalabya 1; Tomas, Ruben M. F.1; Gao, Yanan 2,3 ; Guo, Qiongyu4 ; Gibson, Matthew I.1,2
通讯作者	Gibson, Matthew I.
发表日期	2023
DOI	10.1021/acsami.2c18288
发表期刊	ACS Applied Materials & Interfaces 影响因子和分区
ISSN	1944-8244
EISSN	1944-8252
卷号	15 期号:2
摘要	Spheroids are a powerful tool for basic research and to reduce or replace in vivo (animal) studies but are not routinely banked nor shared. Here, we report the successful cryopreservation of hepatocyte spheroids using macromolecular (polyampholyte) cryoprotectants supplemented into dimethyl sulfoxide (DMSO) solutions. We demonstrate that a polyampholyte significantly increases post-thaw recovery, minimizes membrane damage related to cryo-injury, and remains in the extracellular space making it simple to remove post-thaw. In a model toxicology challenge, the thawed spheroids matched the performance of fresh spheroids. F-actin staining showed that DMSO-only cryopreserved samples had reduced actin polymerization, which the polyampholyte rescued, potentially linked to intracellular ice formation. This work may facilitate access to off-the-shelf and ready-to-use frozen spheroids, without the need for in-house culturing. Readily accessible 3-D cell models may also reduce the number of in vivo experiments.
关键词	macromolecular cryoprotectants polymers spheroids cryopreservation cell-based assays macromolecules ice
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	European Research Council (ERC) under European Union["866056","899872"] ; Royal Society for an Industry Fellowship[191037] ; University of Warwick[BB/W510907/1] ; InnovateUK[10004515] ; National Natural Science Foundation of China[81971764]
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000914744600001
出版者	AMER CHEMICAL SOC
来源库	Web of Science
引用统计	被引频次[WOS]：13
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/475077
专题	工学院_生物医学工程系
作者单位	1.Univ Warwick, Warwick Med Sch, Div Biomed Sci, Coventry CV4 7AL, England 2.Univ Warwick, Dept Chem, Coventry CV4 7AL, England 3.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 4.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Bissoyi, Akalabya,Tomas, Ruben M. F.,Gao, Yanan,et al. Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants[J]. ACS Applied Materials & Interfaces,2023,15(2).
APA	Bissoyi, Akalabya,Tomas, Ruben M. F.,Gao, Yanan,Guo, Qiongyu,&Gibson, Matthew I..(2023).Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants.ACS Applied Materials & Interfaces,15(2).
MLA	Bissoyi, Akalabya,et al."Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants".ACS Applied Materials & Interfaces 15.2(2023).