南方科技大学知识苑(SUSTech KC): PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa

题名	PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa
作者	Cui, Guoyan 1; Zhang, Yixi 2; Xu, Xuejie 1; Liu, Yingying 3; Li, Zhuang 1; Wu, Min 3; Liu, Jianling 1; Gan, Jianhua 2; Liang, Haihua1,4
通讯作者	Liu, Jianling; Gan, Jianhua; Liang, Haihua
发表日期	2022-12-07
DOI	10.1126/sciadv.add4220
发表期刊	Science Advances 影响因子和分区
ISSN	2375-2548
卷号	8 期号:49
摘要	To adapt to changes in environmental cues, Pseudomonas aeruginosa produces an array of virulence factors to survive the host immune responses during infection. Metabolic products contribute to bacterial virulence; however, only a limited number of these signaling receptors have been explored in detail for their ability to modulate virulence in bacteria. Here, we characterize the metabolic pathway of 2-methylcitrate cycle in P. aeruginosa and unveil that PmiR served as a receptor of 2-methylisocitrate (MIC) to govern bacterial virulence. Crystallographic studies and structural-guided mutagenesis uncovered several residues crucial for PmiR's allosteric activation by MIC. We also demonstrated that PmiR directly repressed the pqs quorum-sensing system and subsequently inhibited pyocyanin production. Moreover, mutation of pmiR reduces bacterial survival in a mouse model of acute pneumonia infection. Collectively, this study identified P. aeruginosa PmiR as an important metabolic sensor for regulating expression of bacterial virulence genes to adapt to the harsh environments.
相关链接	[来源记录]
收录类别	SCI
语种	英语
重要成果	NI论文
学校署名	通讯
资助项目	National Natural Science Foundation of China["32170188","31870060"] ; ShaanXi Science and Technology Innovation Team[2019TD-016]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000917811400009
出版者	AMER ASSOC ADVANCEMENT SCIENCE
来源库	Web of Science
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/475091
专题	南方科技大学
作者单位	1.Northwest Univ, Coll Life Sci, Key Lab Resources Biol & Biotechnol Western China, Minist Educ, Xian, Shaanxi, Peoples R China 2.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Collaborat Innovat Ctr Genet & Dev, Sch Life Sci,State Key Lab Genet Engn, Shanghai 200438, Peoples R China 3.Univ North Dakota, Sch Med & Hlth Sci, Dept Biomed Sci, Grand Forks, ND USA 4.Southern Univ Sci & Technol, Coll Med, Shenzhen, Peoples R China
通讯作者单位	南方科技大学
推荐引用方式 GB/T 7714	Cui, Guoyan,Zhang, Yixi,Xu, Xuejie,et al. PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa[J]. Science Advances,2022,8(49).
APA	Cui, Guoyan.,Zhang, Yixi.,Xu, Xuejie.,Liu, Yingying.,Li, Zhuang.,...&Liang, Haihua.(2022).PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa.Science Advances,8(49).
MLA	Cui, Guoyan,et al."PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa".Science Advances 8.49(2022).