A TurboID-based proximity labelling approach for identifying the DNA-binding proteins

Wei，Xia fei1,2; Li，Shan1; Hu，Jie li1

2023-03-17

10.1016/j.xpro.2023.102139

STAR Protocols   影响因子和分区

2666-1667

2666-1667

Biotin proximity labeling is a technique based on the TurboID enzyme that can be used to capture weak or dynamic interactions that had previously not been used to map proteins interacting with a specific DNA sequence. Here, we present a protocol for identifying specific DNA-sequence-binding proteins. We describe steps for biotin labeling of DNA-binding proteins, protein enrichment and sodium dodecyl sulfate polyacrylamide gel electrophoresis separation, and proteomic analysis. For complete details on the use and execution of this protocol, please refer to Wei et al. (2022).

Mass Spectrometry Molecular/Chemical Probes Protein Biochemistry Proteomics

ESCI

英语

其他

National Natural Science Foundation of China["81671997","U20A20392","81902060","cstc2021jcyj-msxmX0298"] ; Natural Science Foundation of Chongqing["cstc2020jcyj-msxmX0764","cstc2020jscx-dxwtBX0022","D20028"] ; 111 Project[202104] ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University[W0049] ; CQMU Program for Youth Innovation in Future Medicine[BJRC201903] ; null[81871635]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:001053554000001

ELSEVIER

2-s2.0-85148771026

Scopus

1.Key Laboratory of Molecular Biology on Infectious Diseases,Ministry of Education,Chongqing Medical University,Chongqing,China
2.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,China

Wei，Xia fei,Li，Shan,Hu，Jie li. A TurboID-based proximity labelling approach for identifying the DNA-binding proteins[J]. STAR Protocols,2023,4(1).

Wei，Xia fei,Li，Shan,&Hu，Jie li.(2023).A TurboID-based proximity labelling approach for identifying the DNA-binding proteins.STAR Protocols,4(1).

Wei，Xia fei,et al."A TurboID-based proximity labelling approach for identifying the DNA-binding proteins".STAR Protocols 4.1(2023).