The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization

Jia, Tianyuan1,2,3; Wu, Pan1,2; Liu, Bin1,2,4,5; Liu, Miaomiao1,2; Mu, Huiqian1,2; Liu, Dan1,2; Huang, Min1,2; Li, Linxing1,2; Wei, Yi1,2; Wang, Lu1,2; Yang, Qian1,2; Liu, Yutao1,2; Yang, Bin1,2,4; Huang, Di1,2,4,5; Yang, Liang3

2023-01-10

10.1126/scisignal.abm0488

SCIENCE SIGNALING   影响因子和分区

1945-0877

1937-9145

Escherichia coli are part of the normal intestinal microbiome, but some enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains can cause potentially life-threatening gastroenteritis. Virulence factors underlying the ability of EHEC and EPEC to cause disease include those encoded in the locus of the enterocyte effacement (LEE) pathogenicity island. Here, we demonstrated that EsrL, a small RNA present in many E. coli strains, promoted pathogenicity, adhesion, and biofilm formation in EHEC and EPEC. PhoB, the response regu-lator of the two-component system that controls cellular responses to phosphate, directly repressed esrL expres-sion under low-phosphate conditions. A phosphate-rich environment, similar to that of the human intestine, relieved PhoB-mediated repression of esrL. EsrL interacted with and stabilized the LEE-encoded regulator (ler) transcript, which encodes a transcription factor for LEE genes, leading to increased bacterial adhesion to cul-tured cells and colonization of the rabbit colon. EsrL also bound to and stabilized the fimC transcript, which encodes a chaperone that is required for the assembly of type 1 pili, resulting in enhanced cell adhesion in pathogenic E. coli and enhanced biofilm formation in pathogenic and nonpathogenic E. coli. Our findings dem-onstrate that EsrL stimulates the expression of virulence genes in both EHEC and EPEC under phosphate-rich conditions, thus promoting the pathogenicity of EHEC and EPEC in the nutrient-rich gut environment.

SCI

英语

其他

National Natural Science Foundation of China Program["32070130","81772148","31820103002","31770144","81871624"] ; Distinguished Young Scholars of Tianjin[20JCJQJC00180] ; Natural Science Foundation (Key Project) of Tianjin[20JCZDJC00820] ; Natural Science Foundation of Shenzhen[JCYJ20220530164604010]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000933631100001

AMER ASSOC ADVANCEMENT SCIENCE

Web of Science

1.Nankai Univ, Inst Translat Med Res, TEDA Inst Biol Sci & Biotechnol, Tianjin Union Med Ctr, Tianjin, Peoples R China
2.Nankai Univ, Key Lab Mol Microbiol & Technol, Minist Educ, Tianjin, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
4.Minist Educ, Ctr Microbial Funct Ge n & Detect Technol, Tianjin, Peoples R China
5.Nankai Int Adv Res Inst, Shenzhen, Peoples R China

Jia, Tianyuan,Wu, Pan,Liu, Bin,et al. The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization[J]. SCIENCE SIGNALING,2023,16(767).

Jia, Tianyuan.,Wu, Pan.,Liu, Bin.,Liu, Miaomiao.,Mu, Huiqian.,...&Yang, Liang.(2023).The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization.SCIENCE SIGNALING,16(767).

Jia, Tianyuan,et al."The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization".SCIENCE SIGNALING 16.767(2023).