Anti-Coronaviral Nanocluster Restrain Infections of SARS-CoV-2 and Associated Mutants through Virucidal Inhibition and 3CL Protease Inactivation

Tang, Hao1; Qin, Hongbo2; He, Shiting2; Li, Qizhen1; Xu, Huan3; Sun, Mengsi3; Li, Jiaan1; Lu, Shanshan2; Luo, Shengdong4; Mao, Panyong4; Han, Pengjun2; Song, Lihua2; Tong, Yigang2; Fan, Huahao2; Jiang, Xingyu1

2023-02-01

10.1002/advs.202207098

ADVANCED SCIENCE   影响因子和分区

2198-3844

Antivirals that can combat coronaviruses, including SARS-CoV-2 and associated mutants, are urgently needed but lacking. Simultaneously targeting the viral physical structure and replication cycle can endow antivirals with sustainable and broad-spectrum anti-coronavirus efficacy, which is difficult to achieve using a single small-molecule antiviral. Thus, a library of nanomaterials on GX_P2V, a SARS-CoV-2-like coronavirus of pangolin origin, is screened and a surface-functionalized gold nanocluster (TMA-GNC) is identified as the top hit. TMA-GNC inhibits transcription- and replication-competent SARS-CoV-2 virus-like particles and all tested pseudoviruses of SARS-CoV-2 variants. TMA-GNC prevents viral dissemination through destroying membrane integrity physically to enable a virucidal effect, interfering with viral replication by inactivating 3CL protease and priming the innate immune system against coronavirus infection. TMA-GNC exhibits biocompatibility and significantly reduces viral titers, inflammation, and pathological injury in lungs and tracheas of GX_P2V-infected hamsters. TMA-GNC may have a role in controlling the COVID-19 pandemic and inhibiting future emerging coronaviruses or variants.

3CL protease antiviral coronavirus nanoclusters virucidal inhibition

SCI ; EI

英语

第一 ; 通讯

National Key R&D Program of China["2020YFA0908900","2018YFA0902600","2022YFC0867500","2021YFF1200100","2021YFF1200800","BWS21J025","20SWAQK22","2020YFA0712102"] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; National Natural Science Foundation of China["22234004","32071390","32101125","82151224"] ; Shenzhen Science and Technology Program["KQTD20190929172743294","JCYJ20200109141231365"] ; Chinese Academy of Sciences["QYZDJ-SSW-SLH039","121D11KYSB20170026"] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191] ; GBA Research Innovation Institute for Nanotechnology[2020GN0110] ; Key Project of Beijing University of Chemical Technology["XK1803-06","XK2020-02"] ; Fundamental Research Funds for Central Universities[BUCTZY2022] ; H&H Global Research and Technology Center[H2021028] ; null[SUSTC-2019-132]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000939713500001

WILEY

20231013675003

Biocompatibility ; Diseases ; Nanoclusters

Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Nanotechnology:761 ; Solid State Physics:933

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China
2.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing 100029, Peoples R China
3.Inst Chem Biol, Shenzhen Bay Lab, Shenzhen 518055, Guangdong, Peoples R China
4.Chinese Peoples Liberat Army Gen Hosp, Med Ctr 5, Beijing 100039, Peoples R China

Tang, Hao,Qin, Hongbo,He, Shiting,et al. Anti-Coronaviral Nanocluster Restrain Infections of SARS-CoV-2 and Associated Mutants through Virucidal Inhibition and 3CL Protease Inactivation[J]. ADVANCED SCIENCE,2023,10(13).

Tang, Hao.,Qin, Hongbo.,He, Shiting.,Li, Qizhen.,Xu, Huan.,...&Jiang, Xingyu.(2023).Anti-Coronaviral Nanocluster Restrain Infections of SARS-CoV-2 and Associated Mutants through Virucidal Inhibition and 3CL Protease Inactivation.ADVANCED SCIENCE,10(13).

Tang, Hao,et al."Anti-Coronaviral Nanocluster Restrain Infections of SARS-CoV-2 and Associated Mutants through Virucidal Inhibition and 3CL Protease Inactivation".ADVANCED SCIENCE 10.13(2023).