Age-related transcriptome changes in Sox2+supporting cells in the mouse cochlea

Cheng, Cheng1,2; Wang, Yunfeng3,4,5; Guo, Luo4,5; Lu, Xiaoling4,5; Zhu, Weijie6; Muhammad, Waqas6,7; Zhang, Liyan6; Lu, Ling1; Gao, Junyan8; Tang, Mingliang6; Chen, Fangyi9; Gao, Xia1; Li, Huawei4,5; Chai, Renjie4,5,6,10,11,12

2019-12-02

10.1186/s13287-019-1437-0

Stem Cell Research & Therapy   影响因子和分区

1757-6512

1757-6512

Background Inner ear supporting cells (SCs) in the neonatal mouse cochlea are a potential source for hair cell (HC) regeneration, but several studies have shown that the regeneration ability of SCs decreases dramatically as mice age and that lost HCs cannot be regenerated in adult mice. To better understand how SCs might be better used to regenerate HCs, it is important to understand how the gene expression profile changes in SCs at different ages. Methods Here, we used Sox2(GFP/+) mice to isolate the Sox2+ SCs at postnatal day (P)3, P7, P14, and P30 via flow cytometry. Next, we used RNA-seq to determine the transcriptome expression profiles of P3, P7, P14, and P30 SCs. To further analyze the relationships between these age-related and differentially expressed genes in Sox2+ SCs, we performed gene ontology (GO) analysis. Results Consistent with previous reports, we also found that the proliferation and HC regeneration ability of isolated Sox2+ SCs significantly decreased as mice aged. We identified numerous genes that are enriched and differentially expressed in Sox2+ SCs at four different postnatal ages, including cell cycle genes, signaling pathway genes, and transcription factors that might be involved in regulating the proliferation and HC differentiation ability of SCs. We thus present a set of genes that might regulate the proliferation and HC regeneration ability of SCs, and these might serve as potential new therapeutic targets for HC regeneration. Conclusions In our research, we found several genes that might play an important role in regulating the proliferation and HC regeneration ability of SCs. These datasets are expected to serve as a resource to provide potential new therapeutic targets for regulating the ability of SCs to regenerate HCs in postnatal mammals.

RNA-seq Proliferation Differentiation Sphere formation Gene expression

SCI

英语

其他

Open Research Fund of the State Key Laboratory of Genetic Engineering, Fudan University[SKLGE1809]

Cell Biology ; Research & Experimental Medicine

Cell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental

WOS:000501147100003

BMC

Web of Science

1.Nanjing Univ, Jiangsu Prov Key Med Discipline Lab, Affiliated Drum Tower Hosp, Dept Otolaryngol Head & Neck Surg,Med Sch, 321 Zhongshan Rd, Nanjing 210008, Jiangsu, Peoples R China
2.Res Inst Otolaryngol, 321 Zhongshan Rd, Nanjing 210008, Jiangsu, Peoples R China
3.Shanghai Fenyang Vis & Audit Ctr, Shanghai, Peoples R China
4.Fudan Univ, ENT Inst, Affiliated Eye & ENT Hosp, NHFPC,Shanghai Engn Res Ctr Cochlear Implant, Room 611,Bldg 9,83 Fenyang Rd, Shanghai 200031, Peoples R China
5.Fudan Univ, NHFPC,Affiliated Eye & ENT Hosp, Shanghai Engn Res Ctr Cochlear Implant,Otorhinola, State Key Lab Med Neurobiol,Key Lab Hearing Med, Room 611,Bldg 9,83 Fenyang Rd, Shanghai 200031, Peoples R China
6.Southeast Univ, MOE Key Lab Dev Genes & Human Dis, State Key Lab Bioelect,Inst Life Sci, Coinnovat Ctr Neuroregenerat,Jiangsu Prov High Te, Nanjing 210096, Jiangsu, Peoples R China
7.Fed Urdu Univ Arts, Dept Biotechnol, Sci & Technol, Gulshan & Lqbal Campus, Karachi, Pakistan
8.Jiangsu Rehabil Res Ctr Hearing & Speech Impairme, Nanjing 210004, Jiangsu, Peoples R China
9.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China
10.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226001, Peoples R China
11.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China
12.Capital Med Univ, Beijing Key Lab Neural Regenerat & Repair, Beijing 100069, Peoples R China

Cheng, Cheng,Wang, Yunfeng,Guo, Luo,et al. Age-related transcriptome changes in Sox2+supporting cells in the mouse cochlea[J]. Stem Cell Research & Therapy,2019,10(1).

Cheng, Cheng.,Wang, Yunfeng.,Guo, Luo.,Lu, Xiaoling.,Zhu, Weijie.,...&Chai, Renjie.(2019).Age-related transcriptome changes in Sox2+supporting cells in the mouse cochlea.Stem Cell Research & Therapy,10(1).

Cheng, Cheng,et al."Age-related transcriptome changes in Sox2+supporting cells in the mouse cochlea".Stem Cell Research & Therapy 10.1(2019).