Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform

Xiao, Guohui1,2; Zhang, Su1; Liang, Zhihang3; Li, Guanqiang4; Fang, Mutong1; Liu, Yaya1; Zhang, Juanjuan1; Ou, Min1; He, Xing1; Zhang, Tianyu2; Zeng, Changchun5; Liu, Lei1; Zhang, Guoliang1,3

2019-11-28

10.1007/s10096-019-03757-y

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES   影响因子和分区

0934-9723

1435-4373

The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify M. abscessus species and subspecies. By designing specific sgRNA probes targeting rpoB and erm(41), we demonstrated that M. abscessus could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical M. abscessus isolates were identified, 18 as M. abscessus subsp. abscessus and 20 as M. abscessus subsp. massiliense. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of M. abscessus species and subspecies.

Mycobacterium abscessus subsp abscessus massiliense bolletii FnCas12a sgRNA probe Identification

SCI

英语

第一 ; 通讯

Sanming Project of Medicine in Shenzhen[SZSM201911009]

Infectious Diseases ; Microbiology

Infectious Diseases ; Microbiology

WOS:000498955400001

SPRINGER

MICROBIOLOGY

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Guangdong Key Lab Emerging Infect Dis, Natl Clin Res Ctr Infect Dis, Shenzhen 518112, Guangdong, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Lab Resp Dis, Guangzhou 510530, Guangdong, Peoples R China
3.Guangdong Med Univ, Sch Basic Med Sci, Dongguan 524023, Peoples R China
4.Chinese Univ Hong Kong, Shenzhen Longgang Peoples Hosp, Dept Lab Med, Shenzhen 518172, Guangdong, Peoples R China
5.Guangdong Med Univ, Shenzhen Longhua Dist Cent Hosp, Dept Lab Med, Shenzhen 518110, Guangdong, Peoples R China

Xiao, Guohui,Zhang, Su,Liang, Zhihang,et al. Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES,2019.

Xiao, Guohui.,Zhang, Su.,Liang, Zhihang.,Li, Guanqiang.,Fang, Mutong.,...&Zhang, Guoliang.(2019).Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform.EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES.

Xiao, Guohui,et al."Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform".EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2019).