Dissecting the human immune system with single cell RNA sequencing technology

Xu, Gang1,2; Liu, Yang1,2; Li, Hanjie3; Liu, Lei1,2; Zhang, Shuye4,5; Zhang, Zheng1,2,6,7

2019-12-04

10.1002/JLB.5MR1019-179R

JOURNAL OF LEUKOCYTE BIOLOGY   影响因子和分区

0741-5400

1938-3673

Single-cell RNA sequencing (scRNA-seq) is a powerful new technology allowing the analysis of transcriptomes from individual cell and is ideally suited to dissect immune cell heterogeneity. ScRNA-seq has already been applied to identify novel immune cell subsets, elaborate cellular differentiation trajectories, and elucidate immunopathogenic mechanisms. Here, we briefly discuss the recent progresses and challenges in the scRNA-seq technology including the workflow, recent applications in immunology, and potential hurdles that need to be overcome. This review will highlight how single cell technology promotes our understanding of human immunology.

cell type identification human immune system single-cell RNA sequencing trajectory inference

SCI

英语

第一 ; 通讯

National Program on Key Basic Research Project[2015CB554300]

Cell Biology ; Hematology ; Immunology

Cell Biology ; Hematology ; Immunology

WOS:000500535200001

WILEY

IMMUNOLOGY

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen Peoples Hosp 3, Inst Hepatol,Natl Clin Res Ctr Infect Dis, Shenzhen 518100, Guangdong, Peoples R China
2.Shenzhen Third Peoples Hosp, Guangdong Key Lab Emerging Infect Dis, Shenzhen, Guangdong, Peoples R China
3.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen, Guangdong, Peoples R China
4.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China
5.Fudan Univ, Inst Biomed Sci, Shanghai 201508, Peoples R China
6.Guangzhou Med Univ, Affiliated Hosp 2, Sch Basic Med Sci, Key Lab Immunol,Sino French Hoffmann Inst, Guangzhou, Guangdong, Peoples R China
7.Guangzhou Med Univ, Affiliated Hosp 2, Guangdong Prov Key Lab Allergy & Clin Immunol, Guangzhou, Guangdong, Peoples R China

Xu, Gang,Liu, Yang,Li, Hanjie,et al. Dissecting the human immune system with single cell RNA sequencing technology[J]. JOURNAL OF LEUKOCYTE BIOLOGY,2019.

Xu, Gang,Liu, Yang,Li, Hanjie,Liu, Lei,Zhang, Shuye,&Zhang, Zheng.(2019).Dissecting the human immune system with single cell RNA sequencing technology.JOURNAL OF LEUKOCYTE BIOLOGY.

Xu, Gang,et al."Dissecting the human immune system with single cell RNA sequencing technology".JOURNAL OF LEUKOCYTE BIOLOGY (2019).