PCL/EUG scaffolds with tunable stiffness can regulate macrophage secretion behavior

Xue, Y. Z. B.1,2,3; Niu, Y. M.2; Tang, B.1,3; Wang, C. M.2

2019-11

10.1016/j.pbiomolbio.2019.05.006

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY   影响因子和分区

0079-6107

Osteoarthritis (OA) is a prevalent joint disorder worldwide. Recent studies suggested that macrophages play an important role in the progression of OA. However, the detailed pathology related to macrophages is still ambiguous, especially where related to mechanotransduction. In this study, polycaprolactone (PCL) and Eucommia Ulmoides Gum (EUG) composite scaffolds were first fabricated by electrospinning. The stiffness of as-fabricated scaffolds was altered by adjusting the PCL-to-EUG ratio. The mechanical properties, structural characteristics and chemical composition of the scaffolds were investigated using various materials characterization techniques. The results show that stiffness of the scaffolds was in the same range as that of cartilage tissues with OA. Confocal microscopy and reverse transcription-polymerase chain reaction (RT-PCR) were performed to investigate the macrophages cultured on the scaffolds. Significant morphological changes of cells were observed on PCL/EUG scaffolds with different stiffness. The expression of inflammatory and fibrosis-related cytokines increases as scaffold stiffness decreases, similar to the trend observed in OA progression. (C) 2019 Elsevier Ltd. All rights reserved.

Eucommia Ulmoides gum Electrospinning Osteoarthritis Macrophage

SCI

英语

第一 ; 通讯

Science and Technology Development Fund, Macao SAR (FDCT)[126/2016/A3]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000501658600003

PERGAMON-ELSEVIER SCIENCE LTD

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen, Guangdong, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
3.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China

Xue, Y. Z. B.,Niu, Y. M.,Tang, B.,et al. PCL/EUG scaffolds with tunable stiffness can regulate macrophage secretion behavior[J]. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY,2019,149:4-11.

Xue, Y. Z. B.,Niu, Y. M.,Tang, B.,&Wang, C. M..(2019).PCL/EUG scaffolds with tunable stiffness can regulate macrophage secretion behavior.PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY,149,4-11.

Xue, Y. Z. B.,et al."PCL/EUG scaffolds with tunable stiffness can regulate macrophage secretion behavior".PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY 149(2019):4-11.