Mesenchymal stem cells adhesion on micro-to-nano-scaled hierarchical ti implants fabricated by powder metallurgy and anodization

Zhao, Dapeng1; Zhang, Yuan1; Nie, Hemin1; Yan, Ming2

10.4028/www.scientific.net/KEM.770.70

2018

1013-9826

KEY ENG MATER

770 KEM

70-79

Xian, China

Trans Tech Publications Ltd

The osseointegration of titanium (Ti) implants highly depends on their surface properties, including roughness, wettability and composition. Ti surfaces with micron-scale roughness have demonstrated more rapid bone apposition compared with polished ones. Besides, Ti implants with nano-structured surface also exhibit better cell adhesion and proliferation behavior. However, the optimal surface for bone regeneration is still unknown, partly due to the difficulty in fabricating surfaces with highly reproducible micron- and nano-topography. In this study, Ti implants with two hierarchies of roughness were fabricated by powder metallurgy, followed by anodization treatment to obtain self-assembled TiO2nanotubes on the micro-roughened surface. X-ray diffraction (XRD), scanning electron microscopy (SEM), 3D Laser Scanning Microscope (3D LSM), and fluorescence microscope were used to investigate the properties of the samples. Ra of the powder metallurgy surface was about 5 μm, while, nanotubes of around 100 nm in diameter were observed after the anodization process. Compared with the polished samples as well as the ones with either micron-scale roughness or nano-structured surfaces, the ones with micro-to-nano-scaled hierarchical topography exhibited lower contact angle, higher protein adsorption and significantly improved mesenchymal stem cells (MSCs) initial adhesion.
© 2018 Trans Tech Publications, Switzerland.

其他

EI

National Natural Science Foundation of China[2015-008] ; National Natural Science Foundation of China[51604104]

20182305280062

Bone ; Cell adhesion ; Cell culture ; Fabrication ; Metals ; Nanotubes ; Powder metallurgy ; Scanning electron microscopy ; Stem cells ; Surface morphology ; Tissue regeneration ; Titanium dioxide ; Titanium powder metallurgy ; Yarn

Bioengineering and Biology:461 ; Powder Metal Products:536.3 ; Nanotechnology:761 ; Inorganic Compounds:804.2 ; Fiber Products:819.4 ; Physical Properties of Gases, Liquids and Solids:931.2

EV Compendex

1.College of Biology, Hunan University, Changsha; 410082, China
2.Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen; 518055, China

Zhao, Dapeng,Zhang, Yuan,Nie, Hemin,et al. Mesenchymal stem cells adhesion on micro-to-nano-scaled hierarchical ti implants fabricated by powder metallurgy and anodization[C]:Trans Tech Publications Ltd,2018:70-79.