一种用于扩增B淋巴细胞免疫组库的引物组及其应用

本发明提供一种用于扩增B淋巴细胞免疫组库的引物组及其应用。所述引物组包括一组正向引物和一组反向引物；所述正向引物从5′端到3′端包括依次相连的接头序列1、条码序列1和针对免疫球蛋白可变区互补决定区3的特异性序列；其中，所述针对免疫球蛋白可变区互补决定区3的特异性序列如SEQ ID NO.1～12中的任意一项所示；所述反向引物从5′端到3′端依次为接头序列2、条码序列2和针对免疫球蛋白基因恒定区的特异性序列。利用所述引物组对IgAN患者和正常对照组的B淋巴细胞进行扩增，收集文库信息，通过测序分析IgAN患者CDR3的长度和多样性变化。

The invention provides a primer group for amplifying a B lymphocyte immune repertoire and an application of the primer group. The primer group comprises a group of forward primers and a group of reverse primers. The forward primers comprise a linker sequence 1, a bar code sequence 1 and a specific sequence aiming at an immunoglobulin variable region complementation determining region 3, which areconnected in sequence from a 5' end to a 3' end, wherein the specific sequence aiming at the immunoglobulin variable region complementary determining region 3 is shown as any one of SEQ ID NO. 1 to SEQ ID NO. 12; and the reverse primers sequentially comprise a linker sequence 2, a bar code sequence 2 and a specific sequence aiming at an immunoglobulin gene constant region from the 5' end to the 3'end. The primer group is used for amplifying B lymphocytes of an IgAN patient and a normal control group, library information is collected, and the length and diversity changes of CDR3 of the IgAN patient are analyzed through sequencing.