On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies

Rani, Andleeb1; Zhao, Yu1; Yan, Qiulin1; Wang, Ying1; Ma, Ran1; Zhu, Zhenyu1; Wang, Bo1; Li, Ting3; Zhou, Xiuwen2; Hocart, Charles H.1,4; Zhou, Youping1,2

2023-03-01

10.1021/acs.analchem.2c04384

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

The 18O/16O ratio of alpha-cellulose in land plants has proved of interest for climate, environmental, physiological, and metabolic studies. Reliable application of such a ratio may be compromised by the presence of hemicellulose impurities in the alpha- cellulose product obtainable with current extraction methods, as the impurities are known to be isotopically different from that of the alpha-cellulose. We first compared the quality of hydrolysates of "alpha- cellulose products" obtained with four representative extraction methods (Jayme and Wise; Brendel; Zhou; Loader) and quantified the hemicellulose-derived non-glucose sugars in the alpha-cellulose products from 40 land grass species using gas chromatography- mass spectrometry (GC/MS). Second, we performed compound specific isotope analysis of the hydrolysates using GC/Pyrolysis/ IRMS. These results were then compared with the bulk isotope analysis using EA/Pyrolysis/IRMS of the alpha-cellulose products. We found that overall, the Zhou method afforded the highest purity alpha-cellulose as judged by the minimal presence of lignin and the second-lowest presence of non-glucose sugars. Isotopic analysis then showed that the O-2-O-6 of the alpha-cellulose glucosyl units were all depleted in 18O by 0.0-4.3 mUr (average, 1.9 mUr) in a species-dependent manner relative to the alpha-cellulose products. The positive isotopic bias of using the alpha-cellulose product instead of the glucosyl units stems mainly from the fact that the pentoses that dominate hemicellulose contamination in the alpha-cellulose product are relatively enriched in 18O (compared to hexoses) as they inherit only the relatively 18O-enriched O-2-O-5 moiety of sucrose, the common precursor of pentoses and hexoses in cellulose, and are further enriched in 18O by the (incomplete) hydrolysis.

SCI ; EI

英语

NI论文

通讯

Chinese MOST[2018YFE0206400] ; Chinese NSF["42250203","41973072","32071507","42007408"]

Chemistry

Chemistry, Analytical

WOS:000946471800001

AMER CHEMICAL SOC

20231113705487

Extraction ; Gas chromatography ; Glucose ; Isotopes ; Mass spectrometry ; Metabolism ; Oxygen ; Physiology

Biology:461.9 ; Chemistry:801 ; Chemical Operations:802.3 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Cellulose, Lignin and Derivatives:811.3 ; Organic Polymers:815.1.1

CHEMISTRY

Web of Science

1.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Isotop Chem Biol ICB, Xian 710021, Peoples R China
2.Southern Univ Sci & Technol, Dept Ocean Sci & Engn, Isotop Chem & Biol Oceanog ICBO, Shenzhen 518055, Peoples R China
3.James Cook Univ, Coll Sci & Engn, ARC Ctr Excellence Australian Biodivers & Heritage, Ctr Trop Environm & Sustainabil Sci, Cairns 4878, Australia
4.Australian Natl Univ, Res Sch Biol, Acton, ACT 2601, Australia

Rani, Andleeb,Zhao, Yu,Yan, Qiulin,et al. On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies[J]. ANALYTICAL CHEMISTRY,2023,95(11):4871-4879.

Rani, Andleeb.,Zhao, Yu.,Yan, Qiulin.,Wang, Ying.,Ma, Ran.,...&Zhou, Youping.(2023).On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies.ANALYTICAL CHEMISTRY,95(11),4871-4879.

Rani, Andleeb,et al."On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies".ANALYTICAL CHEMISTRY 95.11(2023):4871-4879.