南方科技大学知识苑(SUSTech KC): Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems

题名	Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems
作者	Zhang, Junning1 ; Peng, Dinglu1 ; Qin, Wei1 ; Qi, Weizhi1 ; Liu, Xiaoya1 ; Luo, Yucheng1 ; Guo, Qiongyu1 ; Xi, Lei1,2,3
通讯作者	Guo, Qiongyu; Xi, Lei
发表日期	2023-03-01
DOI	10.1002/lpor.202201031
发表期刊	LASER & PHOTONICS REVIEWS 影响因子和分区
ISSN	1863-8880
EISSN	1863-8899
卷号	17 期号:7
摘要	Organs maintain their unique functions through a variety of distinctive vessel systems. However, examination of multiset vessels over the entire organ still remains challenging due to the difficulties of assigning multiple vessel systems and labor-intensive imaging reconstruction technologies. Here, an organ-level photoacoustic microscopic imaging platform, named Organ-PAM, with a micron-scale spatial resolution and ultra-large field of view (FOV), which can achieve a lateral and axial FOV of up to approximate to 81 cm(2) and approximate to 30 mm, respectively, is presented. With the assistance of whole-organ decellularization, optical clearing, and a specifically designed photoacoustic gradient concentration differential angiographic pipeline, the visualization of up to four sets of vessel systems inside diversified organs is successfully achieved with multiple scales. In addition, quantitative analyses of vessel systems in both healthy livers and kidneys as well as cancerous livers with exogenous transplanted tumors are conducted at different stages. Thus, the platform enables high-efficiency multiset vessel imaging, recognition, and quantification of different organs, providing critical insights into distinct vessel systems under varying pathological conditions.
关键词	dual illuminant exogenous transplanted hepatic tumors photoacoustic microscopy whole-organ vessel systems
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Natural Science Foundation of China["62022037","62105140","61775028","81571722","61528401","81971764"] ; Department of Science and Technology of Guangdong Province["2019ZT08Y191","SZBL2020090501013","2022B1212010003"] ; Guangdong Provincial Department of Education[2021ZDZX1064] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Shenzhen Science and Technology Program["KQTD20190929172743294","JCYJ20200109141222892","RCBS20210706092213005","JCYJ20220818101403008"] ; Southern University of Science and Technology[PDJH2021C008] ; Innovation Committee of Shenzhen Municipality[JCYJ20200109141638004]
WOS研究方向	Optics ; Physics
WOS类目	Optics ; Physics, Applied ; Physics, Condensed Matter
WOS记录号	WOS:000952247000001
出版者	WILEY-V C H VERLAG GMBH
来源库	Web of Science
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/523955
专题	工学院_生物医学工程系
作者单位	1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China 3.Shenzhen Bay Lab, Shenzhen 518132, Guangdong, Peoples R China
第一作者单位	生物医学工程系
通讯作者单位	生物医学工程系; 南方科技大学
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Zhang, Junning,Peng, Dinglu,Qin, Wei,et al. Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems[J]. LASER & PHOTONICS REVIEWS,2023,17(7).
APA	Zhang, Junning.,Peng, Dinglu.,Qin, Wei.,Qi, Weizhi.,Liu, Xiaoya.,...&Xi, Lei.(2023).Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems.LASER & PHOTONICS REVIEWS,17(7).
MLA	Zhang, Junning,et al."Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems".LASER & PHOTONICS REVIEWS 17.7(2023).