南方科技大学知识苑(SUSTech KC): RabA2A GTPase Regulates the Secretory Pathway in Parallel to the Exocyst Complex in Arabidopsis

题名	RabA2A GTPase Regulates the Secretory Pathway in Parallel to the Exocyst Complex in Arabidopsis
作者	李瑞熙
报告日期	2022-11-26
会议名称	中国电镜年会
摘要	Delivery of proteins to the plasma membrane occurs via secretion, which requires tethering, docking, priming, and fusion of vesicles. In yeast and mammalian cells, an evolutionarily conserved RAB GTPase activation cascade functions together with the exocyst and SNARE proteins to coordinate vesicle transport with fusion at the plasma membrane. However, whether this is the case in plants is unclear. In this study, we show that the small GTPase RABA2a recruits and interacts with the VAMP721/722-SYP121-SNAP33 SNARE ternary complex for membrane fusion. We also demonstrate that both RABA2a and the downstream SNARE proteins do not interact with the exocyst subunits. RABA2a inactivation does not affect the subcellular localization or assembly of the exocyst, and the mutation of exocyst subunit does not disrupt the RABA2a-SNARE association or SNARE assembly. Therefore, the RABA2a-SNARE- and exocyst-mediated secretory pathways are largely independent. Live videos reveal that the two sets of proteins follow non-overlapping trafficking routes. Genetic evidence support that the two pathways select different cargos. Finally, we demonstrate that the plant-specific RABA2a-SNARE pathway is essential to maintain potassium homeostasis. Our findings imply that highly conserved endomembrane proteins develop plant-specific trafficking mechanisms to accommodate the changing environment.
语种	中文
成果类型	演讲报告
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/531328
专题	生命科学学院_生物系
作者单位	南方科技大学
第一作者单位	南方科技大学
推荐引用方式 GB/T 7714	Li RX. RabA2A GTPase Regulates the Secretory Pathway in Parallel to the Exocyst Complex in Arabidopsis

条目包含的文件
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11-26-2022-瑞熙.pdf（6287KB）	--	--	限制开放	--