Lymphocytes regulate expression of the SARS-CoV-2 cell entry factor ACE2 in the pancreas of T2DM patients

Zhang, Peng1,2; Zheng, Chang-Bo3,4; Liu, Xiao-Yu5; Zhang, Xiaowei6; Huang, Lingyan7; Zeng, Xianhai1,2

2023-04-01

10.1111/dme.15106

DIABETIC MEDICINE   影响因子和分区

0742-3071

1464-5491

Aims: COVID-19 patients with type 2 diabetes mellitus (T2DM) show both poorer clinical outcomes and have an increased risk of death. SARS-CoV-2 virus infection requires simultaneous expression of the SARS-CoV-2 cell entry factors angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine type 2 (TMPRSS2) in the same cell. The aim of the study was to explore the underlying mechanisms of a COVID-19 infection in patients with T2DM.Methods: The distribution and expression of AEC2 and TMPRSS2 in different pancreatic cell types in clinical samples of T2DM patients and diabetic mouse models were analysed by single-cell sequencing, bioinformatics analysis and basic experiments.Results: The results showed that ACE2 and TMPRSS2 are expressed in the ducts of the human pancreas. These findings suggest that SARS-CoV-2 can infect ductal cells in vivo through ACE2 and TMPRSS2. T2DM can promote the co-expression of ACE2 and TMPRSS2 in exocrine ducts, including in the human pancreas. We hypothesize that ACE2 expression levels are associated with increased numbers of lymphocytes in vivo.Conclusions: Increased blood glucose levels are associated with increased ACE2 expression and an increased number of lymphocytes. At the same time, lymphocytes can promote ACE2 expression.

inflammation lymphocytes pancreatic ducts SARS-CoV-2 cell entry factor ACE2 type 2 diabetes mellitus

SCI

英语

其他

Applied Basic Research Programs of Yunnan Provincial Science and Technology Department[202101AT070154] ; Basic and Applied Basic Research Foundation of Guangdong Province[2021A1515010970] ; National Natural Science Foundation of China["82003108","81960662","82200550"] ; Science, Technology and Innovation Commission of Shenzhen Municipality["JCYJ20210324132407019","LGKCYLWS2022002"] ; Shenzhen Key Medical Discipline Construction Fund[SZXK039] ; Open Research Foundation[HXDT-2022-1]

Endocrinology & Metabolism

Endocrinology & Metabolism

WOS:000967683600001

WILEY

CLINICAL MEDICINE

Web of Science

1.Longgang ENT Hosp, Shenzhen 518172, Guangdong, Peoples R China
2.Inst ENT, Shenzhen Key Lab ENT, Shenzhen, Guangdong, Peoples R China
3.Kunming Med Univ, Sch Pharmaceut Sci, Kunming, Yunan, Peoples R China
4.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming, Yunan, Peoples R China
5.Southern Univ Sci & Technol, Sch Med, Shenzhen, Guangdong, Peoples R China
6.Shandong First Med Univ, Dept Gastroenterol, Affiliated Hosp 2, Tai An, Shandong, Peoples R China
7.Gen Hosp Ningxia Med Univ, Pathol Dept, Yinchuan, Ningxia, Peoples R China

Zhang, Peng,Zheng, Chang-Bo,Liu, Xiao-Yu,et al. Lymphocytes regulate expression of the SARS-CoV-2 cell entry factor ACE2 in the pancreas of T2DM patients[J]. DIABETIC MEDICINE,2023,40(10).

Zhang, Peng,Zheng, Chang-Bo,Liu, Xiao-Yu,Zhang, Xiaowei,Huang, Lingyan,&Zeng, Xianhai.(2023).Lymphocytes regulate expression of the SARS-CoV-2 cell entry factor ACE2 in the pancreas of T2DM patients.DIABETIC MEDICINE,40(10).

Zhang, Peng,et al."Lymphocytes regulate expression of the SARS-CoV-2 cell entry factor ACE2 in the pancreas of T2DM patients".DIABETIC MEDICINE 40.10(2023).