南方科技大学知识苑(SUSTech KC): The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method

题名	The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method
作者	Wang, Yirong 1; Chen, Jingchun 1; Du, Jinpan 1; Lei, Liming 2; Zhao, Boxin 3; Bai, Yunpeng 4; Chen, Dong 5; Wang, Xipei 6,7; Chen, Chunbo 1,2,8,9
通讯作者	Wang, Xipei; Chen, Chunbo
发表日期	2023-04-07
DOI	10.1155/2023/6674009
发表期刊	INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY 影响因子和分区
ISSN	1687-8760
EISSN	1687-8779
卷号	2023
摘要	Polymyxin B (PB) is a dose-dependent drug used to treat multidrug-resistantgram-negative bacteria, for which a suitable method is needed to determine clinical samples. A simple, economical, and efficient high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method was developed and validated for polymyxin B1 (PB1), polymyxin B1-Ile (PB1-I), polymyxin B2 (PB2), and polymyxin B3 (PB3) in human plasma. Chromatographic column was Waters BEH C18 column (2.1 x 50 mm, 1.7 mu m). Phase A was water with 0.2% formic acid (FA), and phase B was acetonitrile containing 0.2% FA. The elution method is gradient elutio. The total analysis time was 5 min. The pretreatment method involved protein precipitation using acetonitrile containing 0.2% trifluoroacetic acid and 0.1% FA as the precipitant. The recovery rate was 92-99%. The total quantity of PB1 and PB1-I was measured in the linear range of 100-8000 ng/mL. Simultaneously, the total amounts of PB2 and PB3 were measured in the linear range of 11.9-948.5 ng/mL. This validated method was successfully applied to the pharmacokinetics of PB in critically ill patients.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	National Natural Science Foundation of China[202002030317] ; Guangzhou Science and Technology Program[21202104030001419] ; Guangdong Basic and Applied Basic Research Foundation-Joint Fund of Province and Enterprise[2021S0024] ; null[82172162] ; null[(2020) 24] ; null[200221115835503]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000970694200001
出版者	HINDAWI LTD
来源库	Web of Science
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/536146
专题	南方科技大学第一附属医院
作者单位	1.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China 2.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Dept Intens Care Unit Cardiovasc Surg, Guangzhou, Peoples R China 3.Southern Med Univ, Nanfang Hosp, Dept Pharm, Guangzhou, Peoples R China 4.Maoming Peoples Hosp, Ctr Sci Res, Maoming 525000, Peoples R China 5.South China Univ Technol, Sch Med, Guangzhou 510006, Peoples R China 6.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Res Ctr Med Sci, Guangzhou, Guangdong, Peoples R China 7.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Guangdong Prov Key Lab Clin Pharmacol, Guangzhou, Guangdong, Peoples R China 8.South Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Crit Care Med,Clin Med Coll 2,Affiliated Hosp, Shenzhen 518020, Peoples R China 9.Maoming Peoples Hosp, Dept Emergency, Maoming 525000, Peoples R China
通讯作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Wang, Yirong,Chen, Jingchun,Du, Jinpan,et al. The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method[J]. INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY,2023,2023.
APA	Wang, Yirong.,Chen, Jingchun.,Du, Jinpan.,Lei, Liming.,Zhao, Boxin.,...&Chen, Chunbo.(2023).The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method.INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY,2023.
MLA	Wang, Yirong,et al."The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method".INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY 2023(2023).