Folate Receptor-Targeted NIR-II Dual-Model Nanoprobes for Multiscale Visualization of Macrophages in Rheumatoid Arthritis

Lan, Ruodan1,2; Lv, Jianxun2,3; Gao, Duyang2; Hu, Dehong2; Liu, Chunchen4; Jia, Jie5; Zheng, Hairong2; Liu, Chengbo6; Zhao, Ping5; Sheng, Zonghai2

2023-04-01

10.1002/adfm.202300342

ADVANCED FUNCTIONAL MATERIALS   影响因子和分区

1616-301X

1616-3028

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation. Macrophages accelerate the pathological process of RA through the continuous cell accumulation and secretion of cytokines. Therefore, multiscale visualization of macrophages is of great significance for the early diagnosis of RA. In this work, folate receptor-targeted Cptnc-4F nanoprobes, named FA-CF-NPs, are developed for dual-model molecular imaging of macrophages in RA mouse models. FA-CF-NPs exhibit complementary imaging advantages with high fluorescence sensitivity and resolution in the second near-infrared window (NIR-II) and promising photoacoustic (PA) imaging contrast with deep tissue penetration. The high affinity of the folate ligand on the surface of FA-CF-NPs to macrophages with overexpressed folate receptors ensures the specific targeting function of the nanoprobes for imaging in vivo. FA-CF-NPs are employed to achieve NIR-II fluorescence imaging to accurately localize macrophages in the joint, obtaining a high signal-to-background ratio (SBR) of 15.5. Meanwhile, PA imaging is also performed to observe macrophage distribution in the longitudinal section of the whole joint. Moreover, macrophage infiltration is detected in the joint of RA mice with the absence of RA pathologies such as synovitis and cartilage loss. The results provide an opportunity for in vivo macrophage tracking and early diagnosis of RA.

fluorescence imaging macrophages photoacoustic imaging rheumatoid arthritis second near-infrared windows

SCI ; EI

英语

NI论文

其他

Natural Science Foundation of China["92159304","82171958","81901812","81971638","82027803","81927807","81827807","22204170"] ; CAS Key Laboratory of Health Informatics[2011DP173015] ; Science and Technology Key Project of Shenzhen["JCYJ20190812163614809","JCYJ20200109114612308","JCYJ20210324120011030"] ; Guangdong Basic and Applied Basic Research Fund["2020A1515110011","2022A1515010384"] ; Traditional Chinese Medicine Bureau of Guangdong Province, China[20201114] ; Key Laboratory for Magnetic Resonance and Multimodality Imaging of Guangdong Province[2020B1212060051] ; Natural Science Foundation of Guangdong Province, China[2023A1515012076]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000971358700001

WILEY-V C H VERLAG GMBH

20231713945448

Diagnosis ; Diseases ; Fluorescence imaging ; Infrared devices ; Joints (anatomy) ; Mammals ; Molecular imaging ; Nanoprobes ; Pathology ; Visualization

Biomedical Engineering:461.1 ; Biomechanics, Bionics and Biomimetics:461.3 ; Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Optical Devices and Systems:741.3 ; Imaging Techniques:746 ; Nanotechnology:761 ; Solid State Physics:933

MATERIALS SCIENCE

Web of Science

1.Guangzhou Univ Chinese Med, Guangzhou 510006, Peoples R China
2.Chinese Acad Sci, Inst Biomed & Hlth Engn, Shenzhen Inst Adv Technol, Paul C Lauterbur Res Ctr Biomed Imaging, Shenzhen 518055, Peoples R China
3.Univ Chinese Acad Sci, Shenzhen Coll Adv Technol, Beijing 100049, Peoples R China
4.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen Key Lab Printed Organ Elect, Shenzhen 518055, Peoples R China
5.Guangzhou Univ Chinese Med, Affiliated Hosp 1, Dept Ultrasound, Guangzhou 510405, Peoples R China
6.Chinese Acad Sci, Shenzhen Inst Adv Technol, Res Lab Biomed Opt & Mol Imaging, Guangdong Prov Key Lab Biomed Opt Imaging Technol, Shenzhen 518055, Peoples R China

Lan, Ruodan,Lv, Jianxun,Gao, Duyang,et al. Folate Receptor-Targeted NIR-II Dual-Model Nanoprobes for Multiscale Visualization of Macrophages in Rheumatoid Arthritis[J]. ADVANCED FUNCTIONAL MATERIALS,2023,33(29).

Lan, Ruodan.,Lv, Jianxun.,Gao, Duyang.,Hu, Dehong.,Liu, Chunchen.,...&Sheng, Zonghai.(2023).Folate Receptor-Targeted NIR-II Dual-Model Nanoprobes for Multiscale Visualization of Macrophages in Rheumatoid Arthritis.ADVANCED FUNCTIONAL MATERIALS,33(29).

Lan, Ruodan,et al."Folate Receptor-Targeted NIR-II Dual-Model Nanoprobes for Multiscale Visualization of Macrophages in Rheumatoid Arthritis".ADVANCED FUNCTIONAL MATERIALS 33.29(2023).