Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury

Yang, Qinfeng1; Su, Shenghui2; Liu, Shencai1; Yang, Sheng1; Xu, Jing3; Zhong, Yixiu4; Yang, Yusheng5; Tian, Liangjie5; Tan, Zilin6; Wang, Jian1; Yu, Zhiqiang7,8; Shi, Zhanjun1; Liang, Fangguo9

2023-08-01

10.1016/j.bioactmat.2023.02.024

BIOACTIVE MATERIALS   影响因子和分区

2452-199X

Over the years, electroconductive hydrogels (ECHs) have been extensively applied for stimulating nerve regeneration and restoring locomotor function after peripheral nerve injury (PNI) with diabetes, given their favorable mechanical and electrical properties identical to endogenous nerve tissue. Nevertheless, PNI causes the loss of locomotor function and inflammatory pain, especially in diabetic patients. It has been established that bone marrow stem cells-derived exosomes (BMSCs-Exos) have analgesic, anti-inflammatory and tissue regen-eration properties. Herein, we designed an ECH loaded with BMSCs-Exos (ECH-Exos) electroconductive nerve dressing to treat diabetic PNI to achieve functional recovery and pain relief. Given its potent adhesive and self -healing properties, this laminar dressing is convenient for the treatment of damaged nerve fibers by automati-cally wrapping around them to form a size-matched tube-like structure, avoiding the cumbersome implantation process. Our in vitro studies showed that ECH-Exos could facilitate the attachment and migration of Schwann cells. Meanwhile, Exos in this system could modulate M2 macrophage polarization via the NF-kappa B pathway, thereby attenuating inflammatory pain in diabetic PNI. Additionally, ECH-Exos enhanced myelinated axonal regeneration via the MEK/ERK pathway in vitro and in vivo, consequently ameliorating muscle denervation atrophy and further promoting functional restoration. Our findings suggest that the ECH-Exos system has huge prospects for nerve regeneration, functional restoration and pain relief in patients with diabetic PNI.

Diabetic peripheral nerve injury Exosomes Electroconductive hydrogel Nerve regeneration Pain relief

SCI

英语

其他

President Foundation of Nanfang Hospital, Southern Medical University[2020C024] ; Natural Science Foundation of Fujian Province[2021J011156]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000972177400001

KEAI PUBLISHING LTD

Web of Science

1.Southern Med Univ, Nanfang Hosp, Div Orthopaed Surg, Dept Orthopaed, Guangzhou 510515, Guangdong, Peoples R China
2.Ningde Normal Univ, Ningde Municipal Hosp, Dept Orthopaed, Ningde 352100, Fujian, Peoples R China
3.Southern Med Univ, Nanfang Hosp, Dept Ophthalmol, Guangzhou 510515, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Dermatol, Shenzhen 518020, Guangdong, Peoples R China
5.Southern Med Univ, Nanfang Hosp, Dept Orthopaed, Div Orthopaed & Traumatol, Guangzhou 510515, Guangdong, Peoples R China
6.Southern Med Univ, Nanfang Hosp, Dept Gen Surg, Guangzhou 510515, Guangdong, Peoples R China
7.Southern Med Univ, Integrated Hosp Tradit Chinese Med, Canc Ctr, Guangzhou 510515, Guangdong, Peoples R China
8.Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou 510515, Guangdong, Peoples R China
9.Southern Med Univ, Nanfang Hosp, Dept Plast & Cosmet Surg, Guangzhou 510515, Guangdong, Peoples R China

Yang, Qinfeng,Su, Shenghui,Liu, Shencai,et al. Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury[J]. BIOACTIVE MATERIALS,2023,26.

Yang, Qinfeng.,Su, Shenghui.,Liu, Shencai.,Yang, Sheng.,Xu, Jing.,...&Liang, Fangguo.(2023).Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury.BIOACTIVE MATERIALS,26.

Yang, Qinfeng,et al."Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury".BIOACTIVE MATERIALS 26(2023).