Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

Zhao, Jiankang1,2; Pu, Danni1,2,3; Zhang, Yulin1,2; Qu, Jiuxin4; Lu, Binghuai1,2; Cao, Bin1,2,3,5,6

2023-05-01

10.1128/spectrum.01414-22

MICROBIOLOGY SPECTRUM   影响因子和分区

2165-0497

["Tuberculosis remains a major global public concern as a leading cause of health care-associated infections. The detection of Mycobacterium tuberculosis (MTB) is challenging due to the paucibacillary nature of the pathogen. For suspected pulmonary and extrapulmonary tuberculosis patients, if sputum, bronchoalveolar lavage fluid (BALF), related samples are negative for MTB, or suspected tumors, biopsy tissues may provide a better diagnostic yield. This study was aimed at comparing the performances of three methods in identifying MTB in biopsy tissues, including the Bactec mycobacterial growth indicator tube 960 (MGIT 960) system, the GeneXpert MTB/RIF assay (GeneXpert), and the Bactec Myco/F lytic culture (Myco/F) system. Biopsy samples from 3,209 nonduplicated patients were retrospectively enrolled between January 2018 and September 2021, of which 180 (5.6%) were positive for MTB by at least one method. GeneXpert revealed the highest recovery rate (134/162, 82.7%), followed by MGIT 960 (99/135, 73.3%) and Myco/F (26/143, 18.1%), and the composite positive rate for GeneXpert and MGIT 960 was 96.6% (173/179). Pairwise comparisons were conducted after completion of both tests, and the results showed that Myco/F had significantly lower detection rates than GeneXpert and MGIT 960 (16.4% versus 82.8%, P < 0.001; 14.3% versus 71.4%, P < 0.001). In summary, GeneXpert was the most sensitive and recommended method for MTB detection in biopsy tissues, and the combination of GeneXpert and MGIT 960 could improve the overall diagnostic yield.IMPORTANCE Mycobacterium tuberculosis (MTB) poses a severe threat to public health worldwide. The diagnosis of tuberculosis is challenging due to the low load of the microorganism in samples. Biopsy tissues are sometimes collected via invasive procedures with limited size, and additional samples are often inaccessible. The GeneXpert MTB/RIF assay, Bactec MGIT 960 system, and Bactec Myco/F lytic system have been used in detecting MTB in our laboratory. Here, we evaluated the performances of these three methods in 3,209 biopsy tissues samples to establish a more effective protocol based on clinical requirements. Attempts for a locally optimized protocol should be always made.","Mycobacterium tuberculosis (MTB) poses a severe threat to public health worldwide. The diagnosis of tuberculosis is challenging due to the low load of the microorganism in samples."]

Mycobacterium tuberculosis biopsy tissue GeneXpert MGIT 960 Myco F lytic system F system

SCI

英语

其他

National High Level Hospital Clinical Research Funding[2022-NHLHCRF-LX-01] ; National Major Science and Technology Project for the Control and Prevention of Major Infectious Diseases of China[2017ZX10103004] ; Sanming Project of Medicine in Shenzhen[SZSM201911009]

Microbiology

Microbiology

WOS:000982813500001

AMER SOC MICROBIOLOGY

Web of Science

1.China Japan Friendship Hosp, Ctr Resp Med, Natl Clin Res Ctr Resp Dis, Natl Ctr Resp Med,Dept Pulm & Crit Care Med,Lab Cl, Beijing, Peoples R China
2.Chinese Acad Med Sci, Inst Resp Med, Beijing, Peoples R China
3.Chinese Acad Med Sci, Peking Union Med Coll, Grad Sch, Beijing, Peoples R China
4.Southern Univ Sci & Technol, Hosp Affiliated 2, Shenzhen Peoples Hosp 3, Guangdong Prov Clin Res Ctr Infect Dis TB,Natl Cli, Shenzhen, Guangdong, Peoples R China
5.Tsinghua Univ Peking Univ Joint Ctr Life Sci, Beijing, Peoples R China
6.Capital Med Univ, Dept Resp Med, Beijing, Peoples R China

Zhao, Jiankang,Pu, Danni,Zhang, Yulin,et al. Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study[J]. MICROBIOLOGY SPECTRUM,2023,11(3).

Zhao, Jiankang,Pu, Danni,Zhang, Yulin,Qu, Jiuxin,Lu, Binghuai,&Cao, Bin.(2023).Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study.MICROBIOLOGY SPECTRUM,11(3).

Zhao, Jiankang,et al."Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study".MICROBIOLOGY SPECTRUM 11.3(2023).