Circ_0058058 Drives the Malignant Phenotypes and Immune Evasion of Pancreatic Cancer by the MicroRNA-557-Dependent Regulation of PDL1

Lin, Lin1,7; Xiao, Liang1; Jin, Chang'e2; Qin, Ying3; Deng, Haoyuan4; Li, Meixiang1; Lin, Huan5; Gong, Xuehao6

2022-12-01

10.1097/MPA.0000000000002205

PANCREAS   影响因子和分区

0885-3177

1536-4828

ObjectivesPancreatic cancer (PC) is one of the most deadly malignancies in the world. Recently, circular RNAs play crucial roles in PC progression. However, the functions of circ_0058058 in PC are barely known.MethodsThe expression of circ_0058058, microRNA-557-5p (miR-557), and programmed cell death receptor ligand 1 (PDL1) was detected by quantitative real-time polymerase chain reaction. Functional experiments were implemented to disclose the effect of circ_0058058 deficiency on PC cell proliferation, apoptosis, invasion, angiogenesis, and immune escape. The binding relationship between miR-557 and circ_0058058 or PDL1 was identified by dual-luciferase reporter assay and RNA immunoprecipitation assay. In vivo assay was used to disclose the impact of circ_0058058 silencing on tumor formation in vivo.ResultsCirc_0058058 was highly expressed in PC tissues and cell lines. Knockdown of circ_0058058 repressed cell proliferation, invasion, angiogenesis, and immune escape while contributed to apoptosis in PC cells. Mechanically, circ_0058058 worked as a molecular sponge of miR-557 to regulate PDL1 expression. Moreover, circ_0058058 showed a promotional effect on tumor growth in vivo.ConclusionsOur findings suggested that circ_0058058 served as miR-557 sponge to upregulate PDL1, thereby triggering PC proliferation, invasion, angiogenesis, and immune escape.

pancreatic cancer circ_0058058 miR-557 PDL1

SCI

英语

其他

Shenzhen Science and Technology Innovation Foundation[JCYJ20170413161913429] ; Sanming Project of Medicine in Shenzhen[SZSM201612027]

Gastroenterology & Hepatology

Gastroenterology & Hepatology

WOS:000979053600032

LIPPINCOTT WILLIAMS & WILKINS

CLINICAL MEDICINE

Web of Science

1.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Surg & Oncol, Shenzhen, Peoples R China
2.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Dept Resp & Crit Care Med,Clin M, Shenzhen, Peoples R China
3.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Gastrointestinal Surg, Shenzhen, Peoples R China
4.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Hepatobiliary Surg, Shenzhen, Peoples R China
5.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Vasc Surg, Shenzhen, Peoples R China
6.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Ultrasound, Shenzhen, Peoples R China
7.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Surg & Oncol, 3002 Sungang West Rd, Shenzhen 518035, Guangdong, Peoples R China

Lin, Lin,Xiao, Liang,Jin, Chang'e,et al. Circ_0058058 Drives the Malignant Phenotypes and Immune Evasion of Pancreatic Cancer by the MicroRNA-557-Dependent Regulation of PDL1[J]. PANCREAS,2022,51(10).

Lin, Lin.,Xiao, Liang.,Jin, Chang'e.,Qin, Ying.,Deng, Haoyuan.,...&Gong, Xuehao.(2022).Circ_0058058 Drives the Malignant Phenotypes and Immune Evasion of Pancreatic Cancer by the MicroRNA-557-Dependent Regulation of PDL1.PANCREAS,51(10).

Lin, Lin,et al."Circ_0058058 Drives the Malignant Phenotypes and Immune Evasion of Pancreatic Cancer by the MicroRNA-557-Dependent Regulation of PDL1".PANCREAS 51.10(2022).