Rps6ka2 enhances iMSC chondrogenic differentiation to attenuate knee osteoarthritis through articular cartilage regeneration in mice

Zhang, Juan1,2; Liao, Jin-Qi3; Wen, Li-Ru1; Padhiar, Arshad-Ahmed1; Li, Zhu1; He, Zhong-Yuan4; Wu, Hua-Chuan4; Li, Jian-Feng4; Zhang, Shuai5,6; Zhou, Yan1,3; Pan, Xiao-Hua7; Yang, Jian-Hua8; Zhou, Guang-Qian1

2023-06-30

10.1016/j.bbrc.2023.04.049

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

Articular cartilage (AC) is most susceptible to degeneration in knee osteoarthritis (OA); however, the existing treatments for OA do not target the core link of the pathogenesis-"decreased tissue cell function activity and extracellular matrix (ECM) metabolic disorders" for effective intervention. iMSC hold lower heterogeneity and great promise in biological research and clinical applications. Rps6ka2 may play an important role in the iMSC to treat OA. In this study, the CRISPR/Cas9 gene editing Rps6ka2-/- iMSC were obtained. Effect of Rps6ka2 on iMSC proliferation and chondrogenic differentiation was evaluated in vitro. An OA model was constructed in mice by surgical destabilization of medial meniscus (DMM). The Rps6ka2-/- iMSC and iMSC were injected into the articular cavity twice-weekly for 8 weeks. In vitro experiments showed that Rps6ka2 could promote iMSC proliferation and chondrogenic differentiation. In vivo results further confirmed that Rps6ka2 could improve iMSC viability to promote ECM production to attenuate OA in mice. (c) 2023 Elsevier Inc. All rights reserved.

Knee osteoarthritis Articular cartilage regeneration Rps6ka2 iMSC Chondrogenic differentiation

SCI

英语

其他

National Natural Science Foundation of China["82072480","89201312"] ; Shenzhen Commission of Science and Innovation[JCYJ20220530162206012] ; Sanming Project of Medicine in Shenzhen[SZSM202106019]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000991143200001

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Shenzhen Univ, Hlth Sci Ctr, Dept Med Cell Biol & Genet,Shenzhen Engn Lab Regen, Guangdong Key Lab Genom Stabil & Dis Prevent,Shenz, Shenzhen 518107, Peoples R China
2.Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Endocrinol, Hengyang 421001, Hunan, Peoples R China
3.Lungene Biotech Ltd, Shenzhen 518107, Peoples R China
4.Sun Yat Sen Univ, Affiliated Hosp 7, Dept Orthopaed Surg, Innovat Platform Regenerat & Repair Spinal Cord &, Shenzhen 518107, Peoples R China
5.Southern Univ Sci & Technol, Brain Res Ctr, Shenzhen 518107, Peoples R China
6.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518107, Peoples R China
7.Southern Med Univ, Shenzhen Univ, Affiliated Hosp 2,Dept Orthopaed,Peoples Hosp Shen, Clin Med Coll,Guangdong Med Univ,Sch Clin Med 2, Shenzhen 518107, Peoples R China
8.Chinese Univ Hong Kong, Affiliated Hosp 2, Shenzhen & Longgang Dist Peoples Hosp Shenzhen, Shenzhen 518107, Peoples R China

Zhang, Juan,Liao, Jin-Qi,Wen, Li-Ru,et al. Rps6ka2 enhances iMSC chondrogenic differentiation to attenuate knee osteoarthritis through articular cartilage regeneration in mice[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2023,663.

Zhang, Juan.,Liao, Jin-Qi.,Wen, Li-Ru.,Padhiar, Arshad-Ahmed.,Li, Zhu.,...&Zhou, Guang-Qian.(2023).Rps6ka2 enhances iMSC chondrogenic differentiation to attenuate knee osteoarthritis through articular cartilage regeneration in mice.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,663.

Zhang, Juan,et al."Rps6ka2 enhances iMSC chondrogenic differentiation to attenuate knee osteoarthritis through articular cartilage regeneration in mice".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 663(2023).