Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing

Tong, Renyang1; Luo, Lingjie2; Zhao, Yichao1; Sun, Mingze1; Li, Ronghong1; Zhong, Jianmei1; Chen, Yifan1; Hu, Liuhua1; Li, Zheng1; Shi, Jianfeng1; Lyu, Yuyan1; Hu, Li1; Guo, Xiao1; Liu, Qi1; Shuang, Tian1; Zhang, Chenjie1; Yuan, Ancai1; Sun, Lingyue1; Zhang, Zheng3; Qian, Kun1,4,5; Chen, Lei1; Lin, Wei1; Chen, Alex F. F.6; Wang, Feng2; Pu, Jun1

2023-12-31

10.1080/22221751.2023.2187245

EMERGING MICROBES & INFECTIONS   影响因子和分区

2222-1751

Over 3 billion doses of inactivated vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been administered globally. However, our understanding of the immune cell functional transcription and T cell receptor (TCR)/B cell receptor (BCR) repertoire dynamics following inactivated SARS-CoV-2 vaccination remains poorly understood. Here, we performed single-cell RNA and TCR/BCR sequencing on peripheral blood mononuclear cells at four time points after immunization with the inactivated SARS-CoV-2 vaccine BBIBP-CorV. Our analysis revealed an enrichment of monocytes, central memory CD4(+) T cells, type 2 helper T cells and memory B cells following vaccination. Single-cell TCR-seq and RNA-seq comminating analysis identified a clonal expansion of CD4(+) T cells (but not CD8(+) T cells) following a booster vaccination that corresponded to a decrease in the TCR diversity of central memory CD4(+) T cells and type 2 helper T cells. Importantly, these TCR repertoire changes and CD4(+) T cell differentiation were correlated with the biased VJ gene usage of BCR and the antibody-producing function of B cells post-vaccination. Finally, we compared the functional transcription and repertoire dynamics in immune cells elicited by vaccination and SARS-CoV-2 infection to explore the immune responses under different stimuli. Our data provide novel molecular and cellular evidence for the CD4(+) T cell-dependent antibody response induced by inactivated vaccine BBIBP-CorV. This information is urgently needed to develop new prevention and control strategies for SARS-CoV-2 infection. (ClinicalTrials.gov Identifier: NCT04871932).

Inactivated SARS-CoV-2 vaccine BBIBP-CorV single-cell RNA sequencing single-cell TCR BCR sequencing peripheral blood mononuclear cells

SCI

英语

其他

Immunology ; Infectious Diseases ; Microbiology

Immunology ; Infectious Diseases ; Microbiology

WOS:000983598100001

TAYLOR & FRANCIS LTD

Web of Science

1.Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Immune Therapy Inst, Sch Med,Div Cardiol, Shanghai, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Inst Immunol, Sch Med, Dept Immunol & Microbiol,State Key Lab Oncogenes &, Shanghai, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Sch Med,Natl Clin Res Ctr Infect Dis,Inst Hepatolo, Shenzhen, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China
5.Shanghai Jiao Tong Univ, Med X Res Inst, Shanghai, Peoples R China
6.Shanghai Jiao Tong Univ, Xinhua Hosp, Inst Dev & Regenerat Cardiovasc Med, Sch Med, Shanghai, Peoples R China

Tong, Renyang,Luo, Lingjie,Zhao, Yichao,et al. Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing[J]. EMERGING MICROBES & INFECTIONS,2023,12(1).

Tong, Renyang.,Luo, Lingjie.,Zhao, Yichao.,Sun, Mingze.,Li, Ronghong.,...&Pu, Jun.(2023).Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing.EMERGING MICROBES & INFECTIONS,12(1).

Tong, Renyang,et al."Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing".EMERGING MICROBES & INFECTIONS 12.1(2023).