Serum proteome and metabolome uncover novel biomarkers for the assessment of disease activity and diagnosing of systemic lupus erythematosus

He, Jingquan1; Tang, Donger2; Liu, Dongzhou2; Hong, Xiaoping2; Ma, Chiyu2; Zheng, Fengping2; Zeng, Zhipeng2; Chen, Yumei2; Du, Jie4; Kang, Lin4; Yin, Lianghong3; Lu, Qianjin5; Dai, Yong2

2023-06-01

10.1016/j.clim.2023.109330

CLINICAL IMMUNOLOGY   影响因子和分区

1521-6616

1521-7035

Systemic lupus erythematosus (SLE) is an autoimmune disease affecting thousands of people. There are still no effective biomarkers for SLE diagnosis and disease activity assessment. We performed proteomics and metabolomics analyses of serum from 121 SLE patients and 106 healthy individuals, and identified 90 proteins and 76 metabolites significantly changed. Several apolipoproteins and the metabolite arachidonic acid were significantly associated with disease activity. Apolipoprotein A-IV (APOA4), LysoPC(16:0), punicic acid and stearidonic acid were correlated with renal function. Random forest model using the significantly changed molecules identified 3 proteins including ATRN, THBS1 and SERPINC1, and 5 metabolites including cholesterol, palmitoleoylethanolamide, octadecanamide, palmitamide and linoleoylethanolamide, as potential biomarkers for SLE diagnosis. Those biomarkers were further validated in an independent cohort with high accuracy (AUC = 0.862 and 0.898 for protein and metabolite biomarkers respectively). This unbiased screening has led to the discovery of novel molecules for SLE disease activity assessment and SLE classification.

Systemic lupus erythematosus Proteomics Metabolomics SLEDAI Biomarker

SCI

英语

通讯

Science and Technology Plan of Shenzhen[JCYJ20200109144218597] ; Shenzhen Key Medical Discipline Construction Fund[SZXK011] ; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Spe-cialties[SZGSP001]

Immunology

Immunology

WOS:000983667300001

ACADEMIC PRESS INC ELSEVIER SCIENCE

IMMUNOLOGY

Web of Science

1.Guangzhou Tradit Chinese Med Univ, Shenzhen Tradit Chinese Med Hosp, Forth Clin Med Coll, Dept Radiotherapy, Shenzhen 518033, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Shenzhen Engn Re, Shenzhen 518020, Guangdong, Peoples R China
3.First Affiliated Hosp Jinan Univ, Dept Nephrol, Guangzhou 510630, Peoples R China
4.Biotree, Biotree Metabol Res Ctr, Jiading Dist, Shanghai 201800, Peoples R China
5.Cent South Univ, Xiangya Hosp 2, Dept Dermatol, Hunan Key Lab Med Epigen, Changsha 410011, Hunan, Peoples R China

He, Jingquan,Tang, Donger,Liu, Dongzhou,et al. Serum proteome and metabolome uncover novel biomarkers for the assessment of disease activity and diagnosing of systemic lupus erythematosus[J]. CLINICAL IMMUNOLOGY,2023,251.

He, Jingquan.,Tang, Donger.,Liu, Dongzhou.,Hong, Xiaoping.,Ma, Chiyu.,...&Dai, Yong.(2023).Serum proteome and metabolome uncover novel biomarkers for the assessment of disease activity and diagnosing of systemic lupus erythematosus.CLINICAL IMMUNOLOGY,251.

He, Jingquan,et al."Serum proteome and metabolome uncover novel biomarkers for the assessment of disease activity and diagnosing of systemic lupus erythematosus".CLINICAL IMMUNOLOGY 251(2023).