The histone H2B ubiquitination regulator Wac is essential for plasma cell differentiation

Li, Yuxing1; Ruan, Gui-Xin2; Chen, Wenjing1; Huang, Hengjun1; Zhang, Rui1; Wang, Jing1; Ouyang, Yu1; Zhu, Zhijian1; Meng, Limin1; Wang, Ruisi1; Huo, Jianxin3; Xu, Shengli3,4; Ou, Xijun1

2023-05-01

10.1002/1873-3468.14633

FEBS LETTERS   影响因子和分区

0014-5793

1873-3468

Naive B cells become activated and differentiate into antibody-secreting plasma cells (PCs) when encountering antigens. Here, we reveal that the WW domain-containing adapter protein with coiled-coil (Wac), which is important for histone H2B ubiquitination (ubH2B), is essential for PC differentiation. We demonstrate that B cell-specific Wac knockout mice have severely compromised T cell-dependent and -independent antibody responses. PC differentiation is drastically compromised despite undisturbed germinal center B cell response in the mutant mice. We also observe a significant reduction in global ubH2B in Wac-deficient B cells, which is correlated with downregulated expression of some genes critical for cell metabolism. Thus, our findings demonstrate an essential role of Wac-mediated ubH2B in PC differentiation and shed light on the epigenetic mechanisms underlying this process.

antibody response B cell H2B ubiquitination plasma cell Wac

SCI

英语

第一 ; 通讯

Guangdong Basic and Applied Basic Research Foundation[2020A1515010262] ; Shenzhen Science and Technology Innovation Commission["JCYJ20220530115212028","JCYJ20190809161807432"] ; National Natural Science Foundation of China[32170882]

Biochemistry & Molecular Biology ; Biophysics ; Cell Biology

Biochemistry & Molecular Biology ; Biophysics ; Cell Biology

WOS:000993013800001

WILEY

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China
2.Taizhou Univ, Med Sch, Taizhou, Zhejiang, Peoples R China
3.Agcy Sci Technol & Res, Singapore Immunol Network, Singapore 138648, Singapore
4.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore, Singapore

Li, Yuxing,Ruan, Gui-Xin,Chen, Wenjing,et al. The histone H2B ubiquitination regulator Wac is essential for plasma cell differentiation[J]. FEBS LETTERS,2023,597(13).

Li, Yuxing.,Ruan, Gui-Xin.,Chen, Wenjing.,Huang, Hengjun.,Zhang, Rui.,...&Ou, Xijun.(2023).The histone H2B ubiquitination regulator Wac is essential for plasma cell differentiation.FEBS LETTERS,597(13).

Li, Yuxing,et al."The histone H2B ubiquitination regulator Wac is essential for plasma cell differentiation".FEBS LETTERS 597.13(2023).