CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants

Chen，Zhi1,2; Wu，Chenshuo2,3; Yuan，Yuxuan2; Xie，Zhongjian4; Li，Tianzhong2; Huang，Hao1; Li，Shuang5; Deng，Jiefeng5; Lin，Huiling6; Shi，Zhe7; Li，Chaozhou2; Hao，Yabin2; Tang，Yuxuan8; You，Yuehua9,10; Al-Hartomy，Omar A.11; Wageh，Swelm11; Al-Sehemi，Abdullah G.12,13; Lu，Ruitao18; Zhang，Ling14; Lin，Xuechun15; He，Yaqing16; Zhao，Guojun1; Li，Defa17; Zhang，Han2

2023-12-01

10.1186/s12951-023-01903-5

Journal of Nanobiotechnology   影响因子和分区

1477-3155

Since the end of 2019, a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called COVID-19. Up to date, omicron is the latest variant of concern, and BA.5 is replacing the BA.2 variant to become the main subtype rampaging worldwide. These subtypes harbor an L452R mutation, which increases their transmissibility among vaccinated people. Current methods for identifying SARS-CoV-2 variants are mainly based on polymerase chain reaction (PCR) followed by gene sequencing, making time-consuming processes and expensive instrumentation indispensable. In this study, we developed a rapid and ultrasensitive electrochemical biosensor to achieve the goals of high sensitivity, the ability of distinguishing the variants, and the direct detection of RNAs from viruses simultaneously. We used electrodes made of MXene-AuNP (gold nanoparticle) composites for improved sensitivity and the CRISPR/Cas13a system for high specificity in detecting the single-base L452R mutation in RNAs and clinical samples. Our biosensor will be an excellent supplement to the RT-qPCR method enabling the early diagnosis and quick distinguishment of SARS-CoV-2 Omicron BA.5 and BA.2 variants and more potential variants that might arise in the future.

CRISPR Electrochemical biosensor MXene SARS-CoV-2 BA.5 variant

SCI ; EI

英语

ESI高被引

其他

National Natural Science Foundation of China[12274197];National Natural Science Foundation of China[81870337];Natural Science Foundation of Jiangsu Province[BK20210076];Science, Technology and Innovation Commission of Shenzhen Municipality[JCYJ20220818102618040];Science, Technology and Innovation Commission of Shenzhen Municipality[KCXFZ20201221173413038];

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Nanoscience & Nanotechnology

WOS:000980650200003

BMC

20231914058001

Biosensors ; Diagnosis ; Diseases ; Electrochemical biosensors ; Gold nanoparticles ; Polymerase chain reaction

Biomedical Engineering:461.1 ; Medicine and Pharmacology:461.6 ; Biotechnology:461.8 ; Nanotechnology:761 ; Biochemistry:801.2

BIOLOGY & BIOCHEMISTRY

2-s2.0-85156106287

Scopus

1.Qingyuan People’s Hospital,The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan,Guangdong,511518,China
2.International Collaborative Laboratory of 2D,Materials for Optoelectronics Science and Technology of Ministry of Education,Institute of Microscale Optoelectronics,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen,518060,China
3.Yangtze Delta Region Institute (Huzhou),University of Electronic Science and Technology of China,Huzhou,313001,China
4.Institute of Pediatrics,Shenzhen Children’s Hospital,Institute of Pediatrics,Shenzhen Children’s Hospital,Clinical Medical College of Southern University of Science and Technology,Shenzhen,Guangdong,518038,China
5.College of Pharmacy,Dali University,Dali,671000,China
6.Hengyang Medical College,University of South China,Hengyang,Hunan,421001,China
7.School of Physics & New Energy,Xuzhou University of Technology,Xuzhou,Jiangsu,221018,China
8.Shenzhen Metasensing Tech Limited Company,Shenzhen,518000,China
9.Department of Stomatology,Longhua People’s Hospital Affiliated to Southern Medical University,Shenzhen,518109,China
10.School of Stomatology,Southern Medical University,Guangzhou,510515,China
11.Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah,21589,Saudi Arabia
12.Research Center for Advanced Materials Science (RCAMS),King Khalid University,Abha,P.O. Box 9004,61413,Saudi Arabia
13.Department of Chemistry,College of Science,King Khalid University,Abha,P.O. Box 9004,61413,Saudi Arabia
14.Key Lab of Semiconductor Materials Science,Institute of Semiconductors,Chinese Academy of Sciences,Beijing,100083,China
15.Laboratory of All-Solid-State Light Sources,Institute of Semiconductors,Chinese Academy of Sciences,Beijing,100083,China
16.Shenzhen Center for Disease Control and Prevention,Shenzhen,Guangdong,518055,China
17.Department of Laboratory Medicine,Shenzhen Children’s Hospital,Shenzhen,Guangdong,518038,China
18.Shenzhen International Institute for Biomedical Research,Shenzhen,518110,China

Chen，Zhi,Wu，Chenshuo,Yuan，Yuxuan,et al. CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants[J]. Journal of Nanobiotechnology,2023,21(1).

Chen，Zhi.,Wu，Chenshuo.,Yuan，Yuxuan.,Xie，Zhongjian.,Li，Tianzhong.,...&Zhang，Han.(2023).CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants.Journal of Nanobiotechnology,21(1).

Chen，Zhi,et al."CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants".Journal of Nanobiotechnology 21.1(2023).