题名 | Probiotic-fermented Portulaca oleracea L. alleviated DNFB-induced atopic dermatitis by inhibiting the NF-κB signaling pathway |
作者 | |
通讯作者 | Li,Pan |
发表日期 | 2023-09-15
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DOI | |
发表期刊 | |
ISSN | 0378-8741
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EISSN | 1872-7573
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卷号 | 313 |
摘要 | Ethnopharmacological relevance: Probiotic fermentation is a mild and safe biological method to boost the performance of herbs. Portulaca oleracea L. (PO), with folklore records of purgative, anti-dermatological and anti-epidemic effects, has been demonstrated to possess anti-inflammatory, immunomodulatory, and antioxidant properties. However, the potential of PO for the treatment of atopic dermatitis (AD) has not been sufficiently explored. Aim of study: This study aimed to evaluate the therapeutic benefits of PO and fermented Portulaca oleracea L. (FPO) and explore their intrinsic mechanisms. Methods: By utilizing 2,4-dinitrofluorobenzene-induced AD mice as a model, the histopathology of the lesions was observed using H&E and toluidine blue staining methods; the levels of immunoglobulin E (Ig E), histamine (HIS), and thymic stromal lymphopoietin (TSLP) in serum were measured using ELISA, whereas, the expression of inflammatory cytokines in skin lesion was measured using ELISA and immunohistochemistry experiments. The expression of tumor necrosis factor-α (TNF-α), IKKα, NF-κB mRNA was measured using qPCR; and the expression of TNF-α、p-IKKα, p-IκBα, p-NF-κB was measured using western blotting. Results: Both 20 mg/mL PO and FPO alleviated mast cell infiltration and lesion pathology, reduced serum levels of Ig E, HIS and TSLP, down-regulated the expression of AD-related inflammatory cytokines, such as, TNF-α, interferon-γ, and interleukin-4, and increased filaggrin expression. Furthermore, they inhibited the expression of TNF-α, IKKα, and NF-κB genes and TNF-α, p-IKKα, p-NF-κB and p-IκBα proteins associated with the NF-κB signaling pathway. Conclusions: PO and FPO has a positive therapeutic potential on AD, indicating that it may be employed as alternative therapies for AD. |
关键词 | |
相关链接 | [Scopus记录] |
收录类别 | |
语种 | 英语
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学校署名 | 其他
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资助项目 | Natural Science Foundation of Guangdong Province[2020A1515011268];Research and Development[2020B020226008];
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WOS研究方向 | Plant Sciences
; Pharmacology & Pharmacy
; Integrative & Complementary Medicine
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WOS类目 | Plant Sciences
; Chemistry, Medicinal
; Integrative & Complementary Medicine
; Pharmacology & Pharmacy
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WOS记录号 | WOS:001005095400001
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出版者 | |
ESI学科分类 | PHARMACOLOGY & TOXICOLOGY
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Scopus记录号 | 2-s2.0-85158035231
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来源库 | Scopus
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引用统计 |
被引频次[WOS]:11
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成果类型 | 期刊论文 |
条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/536394 |
专题 | 南方科技大学医学院 |
作者单位 | 1.College of Food Science,South China Agricultural University,Guangzhou,510642,China 2.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China |
推荐引用方式 GB/T 7714 |
Zhao,Wenjun,Zhang,Yuwei,Li,Weijie,et al. Probiotic-fermented Portulaca oleracea L. alleviated DNFB-induced atopic dermatitis by inhibiting the NF-κB signaling pathway[J]. Journal of Ethnopharmacology,2023,313.
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APA |
Zhao,Wenjun.,Zhang,Yuwei.,Li,Weijie.,Hu,Quanzhi.,Huang,Haozhang.,...&Li,Pan.(2023).Probiotic-fermented Portulaca oleracea L. alleviated DNFB-induced atopic dermatitis by inhibiting the NF-κB signaling pathway.Journal of Ethnopharmacology,313.
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MLA |
Zhao,Wenjun,et al."Probiotic-fermented Portulaca oleracea L. alleviated DNFB-induced atopic dermatitis by inhibiting the NF-κB signaling pathway".Journal of Ethnopharmacology 313(2023).
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条目包含的文件 | 条目无相关文件。 |
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